Rapid detection of food borne pathogenic bacteria

食源性致病菌快速检测

• 批准号: 6949172
• 负责人: Simon Bystryak
• 金额: $ 47.44万
• 依托单位: ALLIED INNOVATIVE SYSTEMS, LLC
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-09-15 至 2007-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6949172
• 关键词: Escherichia coli 0157:H7; Escherichia coli infections; Listeria; Listeria infections; agglutination reaction; antibacterial antibody; bacterial antigens; bioengineering /biomedical engineering; biomedical equipment development; biophysics; child (0-11); clinical biomedical equipment; clinical research; diagnosis design /evaluation; diagnosis quality /standard; diagnostic tests; food contamination; human tissue; intermolecular interaction; molecular assembly /self assembly; patient oriented research; portable biomedical equipment; rapid diagnosis; ultrasound

DESCRIPTION (provided by applicant): The objective of this proposal is to develop an ultrasound particle agglutination (UPA) method for determination of pathogenic bacteria Escherichia coli O157:H7 and Listeria monocytogenes in body fluids, food and environmental samples. The UPA assay is based on the use of ultrasound to significantly increase the sensitivity and reduce time of conventional particle agglutination immunoassay. Ultrasound waves are used for both accelerating and monitoring of the agglutination process. The effective testing of bacteria requires methods of analysis that meet a number of challenging criteria. Bacterial detection methods have to be rapid and very sensitive since the presence of even a single pathogenic organism in the body or food may be an infectious dose. The immunoassay we propose to develop will be implemented in a simple, portable and inexpensive device based on the novel patented cylindrical resonator technology. Significantly, the method can be easily adapted to detect other target objects. Preliminary studies confirmed feasibility of the major innovative components of the proposed technology and it is shown that the new method has significant commercial and scientific potential.

描述（申请人提供）：本提案的目的是开发一种超声颗粒凝集（UPA）方法，用于测定体液、食品和环境样品中的致病菌大肠杆菌O157:H7和单核细胞增生李斯特氏菌。 UPA 测定基于超声波的使用，显着提高了传统颗粒凝集免疫测定的灵敏度并缩短了时间。超声波用于加速和监测凝集过程。 细菌的有效测试需要满足许多具有挑战性的标准的分析方法。细菌检测方法必须快速且非常灵敏，因为即使体内或食物中存在单一致病微生物也可能具有感染性剂量。我们建议开发的免疫测定将在基于新型专利圆柱形谐振器技术的简单、便携式且廉价的设备中实施。值得注意的是，该方法可以很容易地适用于检测其他目标对象。初步研究证实了所提出技术的主要创新部分的可行性，并表明该新方法具有巨大的商业和科学潜力。