A new hemagglutination method for blood grouping

一种新的血凝方法

• 批准号: 8454971
• 负责人: Simon Bystryak
• 金额: $ 12.75万
• 依托单位: ALLIED INNOVATIVE SYSTEMS, LLC
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-02-01 至 2014-01-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8454971
• 关键词: Acceleration; Agglutination; Agglutination Tests; Antibodies; Automation; Binding; Biological Assay; Blood; Blood Banks; Blood Screening; Blood Tests; Blood donor; Blood specimen; Blood typing procedure; Businesses; Cells; Centrifugation; Clinical; Clinics and Hospitals; Communities; Complex; D Cells; Detection; Development; Devices; Economics; Erythrocytes; Frequencies; Goals; Grouping; Hemagglutination; Hemagglutination test; Hospitals; Immunoassay; Individual; Isoantibodies; Latex; Legal patent; Licensing; Manuals; Methods; Metric; One-Step dentin bonding system; Optics; Patient Care; Performance; Phase; Process; Public Health; Quantitative Evaluations; Reaction; Reading; Reagent; Research; Sampling; Silicon Dioxide; Step Tests; System; Techniques; Technology; Test Result; Testing; Time; Ultrasonics; Ultrasonography; Validation; Variant; accelerated particle; base; blood group; cost; improved; innovation; instrumentation; method development; new technology; novel; particle; public health relevance; rapid technique

DESCRIPTION (provided by applicant): The long-term goal of the proposed research is to develop a novel quantitative particle agglutination technology for improving the performance of blood typing assays. The conventional hemagglutination blood typing assays have serious drawbacks such as limited sensitivity, long time of incubation, subjectivity and large amount of reagents. It is in this context that we propose to develop a new technology, which is free from the aforementioned limitations of the conventional hemagglutination assays. The proposed immunoassay is a sensitive and rapid method that utilizes inexpensive instrumentation. Significantly, the method presents a general approach for enhancement and detection of agglutination of particles of different origin, including latex, silica microparticles, and biologial cells.

描述（由申请人提供）：所提议研究的长期目标是开发一种新型定量颗粒凝集技术，以提高血型测定的性能。传统的血凝血型分析存在灵敏度有限、孵育时间长、主观性强、试剂用量大等严重缺陷。正是在这种背景下，我们建议开发一种新技术，该技术不受传统血凝测定的上述限制。所提出的免疫测定是一种灵敏且快速的方法，使用廉价的仪器。值得注意的是，该方法提供了增强和检测不同来源颗粒（包括乳胶、二氧化硅微粒和生物细胞）凝集的通用方法。