Core--Embryonic Stem Cell Facility

核心--胚胎干细胞设施

基本信息

批准号：
7497931
负责人：
TIMOTHY J. LEY
金额：
$ 27.4万
依托单位：
WASHINGTON UNIVERSITY
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7497931
关键词：
Alleles American Type Culture Collection Animals Arts Cancer Center Cell Line Cells Charge Chimera organism Chimerism Communities Computer Systems Development Consultations Cytogenetic Analysis DNA Derivation procedure Development ES Cell Line Educational process of instructing Erythrocytes Faculty Freezing Galactosidase Generations Genes Germ Lines Hand Harvest Housing Hybrids Individual Injection of therapeutic agent Internet Karyotype determination procedure Knock-in Mouse Knock-out Laboratories LacZ Genes Learning Measures Methodology Methods Microinjections Mus Mutation Mycoplasma Peripheral Plasmids Process Production Protocols documentation Purpose Quality Control Reagent Research Research Methodology Research Personnel Resistance Resources Role Schedule Schools Screening procedure Secondary to Services Site System Techniques Testing Time Transfection Transgenes Transgenic Mice Universities Update Weaning Week Work base blastocyst congenic cost design embryonic stem cell gain of function homologous recombination hygromycin A interest loss of function member mouse model novel payment peripheral blood recombinase transmission process user-friendly vector

项目摘要

The Embryonic Stem Cell Core (ESC Core) is responsible for: . creating and providing state-of-the-art reagents for the production of targeted mutations in embryonic stem cells. . creating and maintaining quality controlled embryonic stem cell lines from a variety of mouse backgrounds. . teaching the methodology of embryonic stem cell culture and manipulation. The ESC Core also provides consultative services for Siteman Cancer Center (SCC) members when they are designing knock-out or knock-in vectors, and it creates and distributes user-friendly plasmids that facilitate the generation of targeting vectors. The ESC Core also attempts to assist all users with blastocyst injections if injection facilities are not available to that laboratory. By providing a comprehensive service, the ESC Core facilitates the production of gain-of-function and loss-of-function mouse models for SCC faculty.

胚胎干细胞核（ESC核）负责：。创建并提供最先进的试剂，以生产胚胎干细胞中靶向突变。。从各种鼠标创建和维持质量控制的胚胎干细胞系背景。。教授胚胎干细胞培养和操纵的方法。 ESC核心还为现场癌症中心（SCC）成员提供咨询服务，当时他们正在设计淘汰或敲门向量，并创建和分发用户友好的质粒，以促进靶向向量的产生。如果该实验室无法使用注射设施，则ESC核心还试图帮助所有注射胚泡的用户。通过提供全面的服务，ESC核心促进了SCC教师的功能障碍和功能丧失鼠标模型的产生。