Embryonic Stem Cell Core

胚胎干细胞核心

• 批准号: 8709498
• 负责人: TIMOTHY J. LEY
• 金额: $ 7.5万
• 依托单位: WASHINGTON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2013
• 资助国家: 美国
• 起止时间: 2013-09-30 至 2015-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/8709498
• 关键词: Books; Cancer Center; Cancer Center Support Grant; Cancer Etiology; Cell Culture Techniques; Dedications; Development; ES Cell Line; Educational process of instructing; Equipment; Faculty; Generations; Genes; Incubators; Individual; Knock-in Mouse; Knock-out; Laboratories; Maintenance; Malignant Neoplasms; Methodology; Mus; Mutation; Plasmids; Process; Production; Quality Control; Reagent; Research; Research Personnel; Resources; Services; Time; Training; Transfection; Work; cost; design; embryonic stem cell; experience; human disease; loss of function; member; mouse model; user-friendly; vector

The Embryonic Stem Cell (ESC) Core is responsible for: ¿ Creating and providing state-of-the-art reagents for the production of targeted mutations in murine embryonic stem cells. ¿ Creating and maintaining quality-controlled embryonic stem cell lines from a variety of mouse backgrounds. ¿ Teaching the methodology of embryonic stem cell culture and manipulation. The ESC Core provides consultative services for Siteman Cancer Center members when they are designing knock-out or knock-in vectors, and it creates and distributes user-friendly plasmids that facilitate the generation of targeting vectors. By providing a comprehensive service, the ESC Core facilitates the production of gain-offunction and loss-of-function mouse models for Siteman Cancer Center faculty.

胚胎干细胞 (ESC) 核心负责： ¿创造并提供最先进的试剂，用于在小鼠中产生靶向突变 胚胎干细胞。 ¿创建和维护来自各种小鼠的质量受控的胚胎干细胞系 背景。 ¿教授胚胎干细胞培养和操作的方法。 ESC核心为Siteman癌症中心成员在设计时提供咨询服务 敲除或敲入载体，并创建和分发用户友好的质粒，以促进生成 通过提供全面的服务，ESC 核心可促进功能获得的产生。 以及为斯特曼癌症中心教师提供的功能丧失小鼠模型。