ANALYTICAL ULTRACENTRIFUGE PURCHASE: SARS- CORONAVIRUS

分析超速离心机购买：SARS-冠状病毒

• 批准号: 7335161
• 负责人: ROBERT S HODGES
• 金额: $ 6.51万
• 依托单位: UNIVERSITY OF COLORADO DENVER
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-03-01 至 2007-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335161
• 关键词: Coronaviridae; RNA; protein protein interaction; proteins; schools

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): The University of Colorado School of Medicine will purchase an analytical ultracentrifugation instrument with support from the NCRR Shared Instrumentation Grant Program. This instrument is required to meet the growing demand for the accurate and rapid determination of size, shape, association and interactions of macromolecules and complexes. The increased demand results from expansion of the faculty in several departments in the School as well as the establishment of the interdepartmental Program in Biomolecular Structure. The requested instrument will greatly improve the existing resources and will accelerate research in a broad range of systems, including: Analysis of self-association and heteroprotein interactions in the human progesterone receptor (D.L. Bain) Obtaining a mechanistic understanding of viral DNA packaging (C.E. Catalano). De novo design of coiled-coils to understand protein folding and stability and investigations of coiled- coils in native proteins, e.g., motor proteins, transporter of ProP and SARS-coronavirus (R.S. Hodges). Determining functional differences of isoforms of progesterone (K. Horwitz). Examining folding of biologically important RNA molecules including functional RNAs from poliovirus and related viruses, and studying protein-RNA interactions from foot and mouth disease virus (J.S. Kieft). Analysis of protein assembly mechanisms and quantification of strength of protein-protein interactions in cellular replicases (C. McHenry). Other areas of current research also include determination of oligomerization and/or protein-protein interactions in a range of receptors (e.g., B cell complement receptor, glycoproteins of coronavirus, ligand binding proteins of estrogen receptor); the role of ETS transcription factors in epithelial cell development and tumorigenesis; subcellular organization of signal transduction pathways; lymphocyte-specific transcription by early B cell factor; storage and intracellular trafficking of lipids; characterization of tissue plasminogen activator protein; characterization of genetically identified telomerase and end-protection complexes in yeast; and molecular mechanisms of pre-mRNA splicing.

该副本是利用NIH/NCRR资助的共享仪器赠款提供的资源的许多研究子项目之一。子弹和调查员（PI）可能已经从其他NIH来源获得了主要资金，因此可以在其他清晰的条目中代表。列出的机构是赠款，这不一定是调查人员的机构。描述（由申请人提供）：科罗拉多大学医学院将在NCRR共享仪器赠款计划的支持下购买分析性超速离心工具。需要该仪器来满足对大分子和复合物的大小，形状，关联和相互作用的准确和快速确定的不断增长的需求。需求的增加是由于教师在学校的多个部门的扩张以及生物分子结构部门间部门的建立。所请求的仪器将大大改善现有资源，并将在广泛的系统中加速研究，包括：在人孕酮受体（D.L. Bain）中对自我关联和杂蛋白相互作用的分析，以获得对病毒DNA包装的机械理解（C.E. Catalano）。 从头设计的盘绕线圈，以了解天然蛋白质中的蛋白质折叠和稳定性以及对盘绕的线圈的研究，例如运动蛋白，Prop和Sars-Coronavirus（R.S. Hodges）。确定孕酮同工型的功能差异（K. Horwitz）。 检查生物学上重要的RNA分子的折叠，包括脊髓灰质炎病毒和相关病毒的功能性RNA，并研究来自脚和口腔疾病病毒的蛋白RNA相互作用（J.S. Kieft）。 分析蛋白质组装机制和细胞复制蛋白相互作用强度的量化（C. mcHenry）。当前研究的其他领域还包括确定一系列受体（例如B细胞补体受体，冠状病毒的糖蛋白，雌激素受体的配体结合蛋白）的寡聚和/或蛋白质 - 蛋白质相互作用； ETS转录因子在上皮细胞发育和肿瘤发生中的作用；信号转导途径的亚细胞组织；早期B细胞因子的淋巴细胞特异性转录；脂质的储存和细胞内贩运；组织纤溶酶原激活蛋白的表征；酵母中遗传鉴定的端粒酶和末端保护复合物的表征；和前mRNA剪接的分子机制。