Olympus FV 1000 Confocal Microscope for Neurobiology and Cellular Biology Researc

用于神经生物学和细胞生物学研究的奥林巴斯 FV 1000 共焦显微镜

• 批准号: 7047364
• 负责人: FRANCISCO J ALVAREZ
• 金额: $ 32.29万
• 依托单位: WRIGHT STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7047364
• 关键词: anatomy; brain; calcium; central nervous system; community; electron microscopy; erythrocytes; homeostasis; injury; lasers; medicine; microscopy; motivation; neurobiology; neurons; physiology; respiratory function; schools; synapses; university

DESCRIPTION (provided by applicant): This proposal requests funds to purchase an Olympus Fluoview (FV) 1000 Spectral Unit Laser Scanning Confocal Microscope to be utilized by an interactive group of NIH-funded investigators, primarily located in the Department of Anatomy and Physiology at Wright State University. The unit will be also available for shared use with other investigators distributed throughout several other Departments of the Medical School and the College of Science and Mathematics. This new confocal system will serve two major purposes, 1) replacement of our six year-old Olympus FX and 2) upgrade our confocal instrumentation capabilities. Major advances provided by the new system include a) multiple fluorochrome labeling beyond our current two channel limitations; b) optimal controls of laser illumination; c) improved fluorochromes separation, even when emission curves are closely related and d) improved file handling, save and export for further quantitative analyses in 2D and 3D space. The instrument will be mostly used by a core group of five investigators that will form a collaborative unit with shared projects and common scientific interests on the modification of synapses and circuits during development, injury and recovery from injury. They have also accumulated a broad range of expertise with imaging equipment and quantitative methods, many of which require the improved capabilities of the Olympus FV 1000 system. The research topics from further users are varied and range from volume or pH control in neurons to cellular calcium transport mechanisms and chloride homeostasis in red blood cells and neurons to central nervous system control of respiratory function. The majority of investigators (major or minor users) are funded through NIH. To promote maintenance and equitable use, the instrument will be integrated within the microscopy and imaging facilities of the Center for Brain Research. This core facility was established in 2000 with the mission of providing imaging and histological needs (electron microscopy, image analysis, neuron tracing, confocal microscopy, microtomy and ultramicrotomy) to researchers located primarily (but not exclusively) in the School of Medicine and with an emphasis in Neuroscience research. As such, the acquisition of the Olympus FV 1000 systems will significantly enhance the research programs of the core investigators, but it will also have a beneficial impact on the broader research community at Wright State University.

描述（由申请人提供）：该提案要求资金购买奥林巴斯 Fluoview (FV) 1000 光谱单元激光扫描共焦显微镜，供 NIH 资助的研究人员互动小组使用，该小组主要位于赖特解剖学和生理学系州立大学。该装置还可与分布在医学院其他几个系以及科学与数学学院的其他研究人员共享使用。这种新的共焦系统将有两个主要用途，1）替换我们已有六年历史的奥林巴斯 FX，2）升级我们的共焦仪器功能。新系统提供的主要进步包括：a) 超越我们当前的两个通道限制的多重荧光染料标记； b) 激光照明的优化控制； c) 改进了荧光染料分离，即使发射曲线密切相关也是如此；d) 改进了文件处理、保存和导出，以便在 2D 和 3D 空间中进行进一步定量分析。该仪器将主要由由五名研究人员组成的核心小组使用，他们将形成一个协作单位，在发育、损伤和损伤恢复过程中突触和电路的修改方面拥有共同的项目和共同的科学兴趣。他们还积累了成像设备和定量方法方面的广泛专业知识，其中许多需要奥林巴斯 FV 1000 系统的改进功能。其他用户的研究主题多种多样，从神经元的体积或 pH 控制到细胞钙转运机制和红细胞和神经元中的氯稳态，再到中枢神经系统对呼吸功能的控制。大多数研究人员（主要或次要用户）均由 NIH 资助。为了促进维护和公平使用，该仪器将集成到脑研究中心的显微镜和成像设施中。该核心设施成立于 2000 年，其使命是为主要（但不限于）医学院的研究人员提供成像和组织学需求（电子显微镜、图像分析、神经元追踪、共聚焦显微镜、切片术和超薄切片术）。重点关注神经科学研究。因此，收购奥林巴斯 FV 1000 系统将显着增强核心研究人员的研究项目，同时也将对莱特州立大学更广泛的研究社区产生有益的影响。