ZEISS LSM 510 META CONFOCAL MICROSOPE: CANCER

ZEISS LSM 510 META 共焦显微镜：癌症

• 批准号: 7335230
• 负责人: Mark David Rose
• 金额: $ 5万
• 依托单位: PRINCETON UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-15 至 2007-04-14
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7335230
• 关键词: fluorescent dye /probe; ionophores; lighting; neoplasm /cancer; particle; proteins; university

This subproject is one of many research subprojects utilizing the resources provided by a Shared Instrumentation Grant funded by NIH/NCRR. The subproject and investigator (PI) may have received primary funding from another NIH source, and thus could be represented in other CRISP entries. The institution listed is for the grant, which is not necessarily the institution for the investigator. DESCRIPTION (provided by applicant): This application requests funds to purchase a Zeiss LSM 510 Meta confocal microscope system. This new instrument will greatly extend the current capabilities for fluorescent microscopy at Princeton University. The Zeiss LSM 510 Meta system will allow researchers to image cells simultaneously labeled with multiple fluorescent probes, including fluorescent dyes and proteins whose emission spectra substantially overlap. Existing microscopes at Princeton University use optical filters and dichroic mirrors to select specific emitted wavelengths and therefore are limited to cleanly distinguishing only 2 different fluorescent proteins in the same cell (e.g. YFP and CFP, or GFP and RFP). Furthermore, when colocalizing fluorescent proteins are imaged on moving particles, spatial offsets occur because the different wavelengths are captured in temporally separate scans/exposures. Both problems lead to limitations in the scope and rate of research progress. The Zeiss LSM 510 Meta detector system solves these problems by using a diffraction grating to spread the emitted light over an array of light detectors, effectively determining the emission spectrum for every pixel in the specimen. Using previously measured reference spectra, the contribution of emitted light from each fluorophore is computed for every point in the image, allowing the spatial distribution of each fluorophore to be imaged separately. Preliminary results with biological systems currently under study at Princeton University, and published results from elsewhere, demonstrate that the Zeiss LSM 510 Meta detector will allow researchers to simultaneously image as many as eight different fluorescent proteins and dyes. This new capability will allow Princeton researchers to identify multiple cell compartments in living cells and follow the transport of rapidly moving intracellular particles. Research currently undertaken at Princeton which will be benefited greatly by a Meta system include studies of mRNA transport and cell movement in Drosophila oocytes and embryos, herpes virus assembly and transport in neuronal cells, morphogenesis of normal and cancerous ducts in breast tissue, C. elegans morphogenesis, and the cell biology of conjugation in yeast. Study of each of these biological systems will provide valuable information pertaining to significant human health problems such as birth defects, viral infection and cancer.

该子项目是利用 NIH/NCRR 资助的共享仪器补助金提供的资源的众多研究子项目之一。子项目和研究者 (PI) 可能已从另一个 NIH 来源获得主要资金，因此可以在其他 CRISP 条目中得到体现。列出的机构是资助机构，不一定是研究者的机构。描述（由申请人提供）：本申请要求资金购买 Zeiss LSM 510 Meta 共焦显微镜系统。这种新仪器将极大地扩展普林斯顿大学当前的荧光显微镜能力。蔡司 LSM 510 Meta 系统将允许研究人员对同时标记有多个荧光探针的细胞进行成像，这些探针包括发射光谱基本重叠的荧光染料和蛋白质。普林斯顿大学现有的显微镜使用滤光片和二向色镜来选择特定的发射波长，因此仅限于清晰地区分同一细胞中的 2 种不同荧光蛋白（例如 YFP 和 CFP，或 GFP 和 RFP）。此外，当共定位荧光蛋白在移动粒子上成像时，会发生空间偏移，因为在时间上独立的扫描/曝光中捕获不同的波长。这两个问题都导致研究进展的范围和速度受到限制。 Zeiss LSM 510 Meta 探测器系统通过使用衍射光栅将发射的光散布到光探测器阵列上，从而有效地确定样本中每个像素的发射光谱，从而解决了这些问题。使用之前测量的参考光谱，可以计算图像中每个点的每个荧光团发射光的贡献，从而可以单独对每个荧光团的空间分布进行成像。普林斯顿大学目前正在研究的生物系统的初步结果以及其他地方发表的结果表明，蔡司 LSM 510 Meta 探测器将允许研究人员同时对多达八种不同的荧光蛋白和染料进行成像。这项新功能将使普林斯顿大学的研究人员能够识别活细胞中的多个细胞区室，并跟踪快速移动的细胞内颗粒的运输。普林斯顿大学目前正在进行的研究将大大受益于 Meta 系统，包括果蝇卵母细胞和胚胎中 mRNA 运输和细胞运动的研究、神经元细胞中疱疹病毒的组装和运输、乳腺组织中正常和癌性导管的形态发生、秀丽隐杆线虫的研究形态发生，以及酵母接合的细胞生物学。对这些生物系统的研究将为出生缺陷、病毒感染和癌症等重大人类健康问题提供有价值的信息。