Receptor Specifcity in the Proliferation and Differentiation of Stem Cells

干细胞增殖和分化中的受体特异性

• 批准号: 7076455
• 负责人: C. Anthony Blau
• 金额: $ 31.88万
• 依托单位: UNIVERSITY OF WASHINGTON
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-05-01 至 2010-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7076455
• 关键词: NOD mouse; SCID mouse; biological signal transduction; biotechnology; cell differentiation; cell proliferation; chemical binding; dogs; embryonic stem cell; fibroblast growth factor; flow cytometry; gene therapy; genetic manipulation; genetically modified animals; growth factor receptors; hematopoiesis; hematopoietic stem cells; hematopoietic tissue transplantation; human embryonic stem cell line; human tissue; laboratory mouse; protein structure function; receptor binding; stem cell transplantation; tissue /cell culture

DESCRIPTION (provided by applicant): The goal of this application is to test a candidate "universal" cell growth switch, a conditional derivative of the fibroblast growth factor receptor 1, F36VFGFR1, in preclinical models of gene and cell therapy. In specific aim 1, we will test whether different growth factor receptor signaling domains differentially regulate human hemopoiesis. In specific aim 2 we will compare the nature, intensity and duration of the hematopoietic response to F36VFGFR1 signaling versus F36Vmp1 signaling in dogs. In specific aim 3 we will test whether F36VFGFR1 signaling promotes self-renewal in engineered human embryonic stem (ES) cells. We will be using the human embryonic stem cell line WA01.

描述（由申请人提供）：本申请的目的是在基因和细胞治疗的临床前模型中测试候选“通用”细胞生长开关，即成纤维细胞生长因子受体 1 F36VFGFR1 的条件衍生物。在具体目标1中，我们将测试不同的生长因子受体信号传导域是否差异性地调节人类造血。在具体目标 2 中，我们将比较狗对 F36VFGFR1 信号传导与 F36Vmp1 信号传导的造血反应的性质、强度和持续时间。在具体目标 3 中，我们将测试 F36VFGFR1 信号传导是否促进工程化人类胚胎干 (ES) 细胞的自我更新。我们将使用人类胚胎干细胞系 WA01。