Myofilament Calcium Sensitivity in Health and Disease

健康和疾病中的肌丝钙敏感性

• 批准号: 7073517
• 负责人: PAULUS ML JANSSEN
• 金额: $ 10.27万
• 依托单位: OHIO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-06 至 2011-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7073517
• 关键词: calcium flux; calcium indicator; calcium transporting ATPase; heart contraction; heart rate; laboratory rabbit; microfilaments; muscle relaxation; sarcoplasmic reticulum; tissue /cell culture

DESCRIPTION (provided by applicant): Applicant: I am an assistant professor in the Department of Physiology and Cell Biology at The Ohio State University. I currently have a teaching load equal to 30-35% effort, and a heavy service load; chair of graduate studies and 3 other dept. committees, recruitment member for our college-wide graduate program and secretary/treasurer of the Cardiovascular System Dynamics society. Upon award, my research time will more than double, and 90%+ off my total effort will be protected for research. Environment: The Ohio State University has provided me with adequate lab-space. I currently hold an AHA SDG, and a NHLBI RO1 to support my research. There is ample opportunity for scientific collaboration, and a wide array of core-facilities are at my disposal, including proteomics-, imaging-, and transgenic facilities. Research Plan: In plan to active participation in 2 weekly seminar series and 1 cardiovascular journal club, while official courses in responsible research practices will be taken. I will also be trained in small animal surgery, and do an internship in our departments' proteomics core facility. The research plan consists of three sections. In the first, I outline a summary of my currently funded RO1. As meanwhile we have made significant progress, in the second section I expanded that project with another aim: to elucidate the effects of myofilament responsiveness during various cardiac pathologies, using the methods and control data obtained with experiments covered in my RO1. In the third section, I proposed an additional line of research, that is related to the RO1 and its' expansion aims in methodological approaches, but has a distinct separate hypothesis. We postulate that heart rate variability (HRV; extensively and exclusively thought of as a marker for cardiac function), is actually a co-determinant of cardiac contractility. We have conducted preliminary experiments to show proof-of-principle of this novel concept, and will further investigate direct and indirect effects of HRV on cardiac function, including a possible novel therapeutic approach for cardiac failure.

描述（由申请人提供）： 申请人：我是俄亥俄州立大学生理学和细胞生物学系的助理教授。我目前的教学负荷等于30-35％的努力，而繁重的服务负荷；研究生研究主席和其他3个部门。委员会，我们在大学范围的研究生课程的招聘会员以及心血管系统动力学协会的秘书/司库。奖励后，我的研究时间将增加两倍以上，而我的总努力将受到90％以上的折扣。环境：俄亥俄州立大学为我提供了足够的实验室空间。我目前持有AHA可持续发展目标和NHLBI RO1，以支持我的研究。科学合作有足够的机会，我可以掌握各种各样的核心功能，包括蛋白质组学 - 成像和转基因设施。研究计划：计划积极参与2周的研讨会系列和1个心血管期刊俱乐部，同时将学习负责任的研究实践的官方课程。我还将接受小型动物手术的培训，并在我们部门的蛋白质组学核心设施中实习。研究计划包括三个部分。首先，我概述了当前资助的RO1的摘要。与此同时，我们在第二部分中取得了重大进展，我使用RO1中涵盖的实验获得的方法和控制数据，以另一个目的扩展了该项目：阐明各种心脏病理中肌丝反应性的影响。在第三部分中，我提出了一项额外的研究线，该研究与RO1及其扩展有关，目的是在方法论方法中，但具有独立的假设。我们假设心率变异性（HRV；广泛，专门将其视为心脏功能的标记），实际上是心脏收缩的共同确定性。我们已经进行了初步实验，以展示这一新颖概念的原则证明，并将进一步研究HRV对心脏功能的直接和间接影响，包括可能导致心脏衰竭的新型治疗方法。