Calcium Buffering Mechanisms in Taste Cells

味觉细胞中的钙缓冲机制

• 批准号: 7065593
• 负责人: KATHRYN MEDLER
• 金额: $ 7.74万
• 依托单位: STATE UNIVERSITY OF NEW YORK AT BUFFALO
• 依托单位国家: 美国
• 财政年份: 2004
• 资助国家: 美国
• 起止时间: 2004-07-01 至 2009-05-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7065593
• 关键词: calcium channel; calcium flux; calcium indicator; calcium transporting ATPase; cell cell interaction; cell population study; cell type; gene expression; laboratory mouse; membrane transport proteins; mitochondria; polymerase chain reaction; sensory feedback; taste; taste buds

DESCRIPTION (provided by applicant): Calcium is an important second messenger for many cellular processes; therefore, its presence is tightly regulated within cells. Cells have developed at least five calcium buffering mechanisms: the plasma membrane Ca2+-ATPase and Na+/Ca2+ exchanger, the Ca2+-ATPase on the endoplasmic reticulum, the mitochondria, and cytosolic calcium buffering proteins. Since a rise in calcium is the final step in taste transduction, the presence of calcium must be tightly regulated in taste cells as well; however, the calcium buffering mechanisms used by taste cells are not known. It is likely that different calcium buffering mechanisms contribute to the formation and modulation of the calcium signal in response to taste stimuli. This is probably critical in determining how a taste cell communicates to other cells. This application intends to characterize the calcium buffering mechanisms that are present in taste cells. It will correlate their expression with the different cell types that are present in taste buds. It has been shown previously that there are two distinct electrophysiological profiles in taste cells, those with voltage gated calcium channels and those without. These two different profiles correlate loosely with cell type. The first hypothesis is that there will be different calcium buffering mechanisms, depending on whether the cells express calcium channels or not. The second hypothesis is that calcium buffering mechanisms are important components in the formation of the cell's response to taste stimuli. The specific aims of this application are: (1) Characterize the expression of calcium buffering mechanisms present in taste cells and correlate their expression to different cell types; (2) Determine the physiological role of calcium buffering mechanisms in taste cells and determine their role in shaping the Ca2+ response to taste stimuli. These aims will be accomplished by using molecular techniques for aim 1 and calcium imaging for aim 2. Results from the proposed experiments will provide new information about how taste cells generate Ca2+ signals and communicate to other cells/afferent neurons. These data will determine if there are differences between taste cells that have calcium influx through voltage gated calcium channels and cells that use the PLC signaling pathway that results in calcium release from intracellular stores. This data will further our understanding about how taste cells generate a signal in response to a taste stimuli.

描述（由申请人提供）：钙是许多蜂窝过程的重要第二信使；因此，其存在在细胞内受到严格调节。细胞已经开发了至少五种钙缓冲机制：质膜Ca2+-ATPase和Na+/Ca2+交换器，内质网上的Ca2+-ATPase，线粒体和胞质钙钙缓冲蛋白。由于钙的升高是味觉转导的最后一步，因此钙的存在也必须在味觉细胞中受到严格调节。但是，尚不清楚味觉细胞使用的钙缓冲机制。不同的钙缓冲机制可能有助于响应味觉刺激的钙信号的形成和调节。这对于确定味觉细胞如何与其他细胞通信可能至关重要。该应用程序旨在表征味觉细胞中存在的钙缓冲机制。它将与味蕾中存在的不同细胞类型相关联。先前已经证明，味觉细胞中有两个不同的电生理谱，具有电压门控钙通道的电池和没有两个不同的电生理谱。这两个不同的轮廓与细胞类型松散相关。第一个假设是，会有不同的钙缓冲机制，具体取决于细胞是否表达钙通道。第二个假设是，钙缓冲机制是形成细胞对味觉刺激的反应的重要组成部分。该应用的具体目的是：（1）表征味道细胞中存在的钙缓冲机制的表达，并将其表达与不同的细胞类型相关联； （2）确定钙缓冲机制在味觉细胞中的生理作用，并确定它们在塑造Ca2+对味觉刺激反应的作用。这些目标将通过使用分子技术来实现AIM 1和AIM 2的钙成像。拟议的实验的结果将提供有关味觉细胞如何产生Ca2+信号并与其他细胞/传播神经元通信的新信息。这些数据将确定通过使用电压门控钙通道流入钙的味道和使用PLC信号传导途径的细胞之间是否存在差异，从而导致细胞内储存的钙释放。这些数据将进一步了解味觉细胞如何响应味觉刺激的味道产生信号。

项目成果

Sodium/calcium exchangers selectively regulate calcium signaling in mouse taste receptor cells.

• DOI: 10.1152/jn.00118.2010
• 发表时间: 2010-05
• 期刊: Journal of neurophysiology
• 影响因子: 2.5
• 作者: Steven A. Szebenyi;Agnieszka I. Laskowski;K. Medler

Calcium signaling in taste cells: regulation required.

• DOI: 10.1093/chemse/bjq082
• 发表时间: 2010-11
• 期刊: Chemical senses
• 影响因子: 3.5
• 作者: K. Medler

Expression of calcium binding proteins in mouse type II taste cells.

小鼠 II 型味觉细胞中钙结合蛋白的表达。

• DOI: 10.1369/0022155411402352
• 发表时间: 2011
• 期刊: The journal of histochemistry and cytochemistry : official journal of the Histochemistry Society
• 作者: Rebello,MichelleR;Aktas,Adem;Medler,KathrynF
• 通讯作者: Medler,KathrynF

Ryanodine receptors selectively contribute to the formation of taste-evoked calcium signals in mouse taste cells.

• DOI: 10.1111/j.1460-9568.2010.07463.x
• 发表时间: 2010-12
• 期刊: The European journal of neuroscience
• 作者: Rebello MR;Medler KF
• 通讯作者: Medler KF

Expression of GABAergic receptors in mouse taste receptor cells.

• DOI: 10.1371/journal.pone.0013639
• 发表时间: 2010-10-26
• 期刊: PloS one
• 影响因子: 3.7
• 作者: Starostik MR;Rebello MR;Cotter KA;Kulik A;Medler KF
• 通讯作者: Medler KF