Mechanisms of MeCP2 gene expression regulation

MeCP2基因表达调控机制

基本信息

批准号：
7184526
负责人：
CAROL S LUTZ
金额：
$ 7.79万
依托单位：
UNIV OF MED/DENT OF NJ-NJ MEDICAL SCHOOL
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-03-15 至 2009-02-28
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7184526
关键词：
3&apos Untranslated Regions 5&apos Untranslated Regions Accounting Address Affect Age-Months Area Attention Autistic Disorder Binding Binding Proteins Bioinformatics Brain Candidate Disease Gene Cell Line Chromatin Chromosome Mapping Code Conserved Sequence Development Diagnosis Disease Elements Exclusion Exons Female Figs - dietary Frequencies Functional RNA Gene Expression Gene Expression Regulation Genes Genetic Transcription Goals Hand In Vitro Incidence Individual Laboratories Link Messenger RNA Methyl-CpG-Binding Protein 2 Microcephaly Modeling Mus Mutation Names Neurodevelopmental Disorder Neurons Open Reading Frames Patients Phenotype Play Poly A Polyadenylation Principal Investigator Process Protein Isoforms RNA RNA Stability Regulation Reporter Reporting Research Research Personnel Rett Syndrome Role Schizophrenia Signal Transduction Speech Symptoms Syndrome System Tissues Transcript Translations Untranslated Regions Work Xq28 base in vivo insight knockout gene mRNA Stability novel relating to nervous system size transmission process

项目摘要

DESCRIPTION (provided by applicant): The goal of this application is to determine the post-transcriptional mechanisms of gene expression control of the MeCP2 gene. The principal investigator will delineate alternative polyadenylation of MeCP2, the process that determines the size of the 3' UTR in a tissue-and developmentally-specific fashion. She will also investigate the role that mRNA stability may play in MeCP2 expression regulation. These studies will be performed in vivo in a variety of cell lines with special attention to neural cells. The investigator will then turn to established in vitro systems using these cell lines in order to investigate mechanisms of regulation by alternative polyadenylation and differential RNA stability. These studies represent an under-explored area of research on MeCP2 gene expression that may have a critical influence in the tissue-specific effects observed in Rett syndrome.

描述（由申请人提供）：本申请的目的是确定MECP2基因基因表达控制的转录后机制。主要研究者将描述MECP2的替代聚腺苷酸化，该过程决定了在组织和发育特定的方式中确定3'UTR的大小。她还将调查mRNA稳定性在MECP2表达调控中的作用。这些研究将在各种细胞系中在体内进行，并特别注意神经细胞。然后，研究者将使用这些细胞系转向已建立的体外系统，以研究通过替代聚腺苷酸化和差异RNA稳定性调节机制。这些研究代表了关于MECP2基因表达的研究不足的研究领域，该研究可能对RETT综合征中观察到的组织特异性作用产生关键影响。