Joint U.S.-India Study of Lactobacillus and its Phages in Bacterial Vaginosis

美印联合研究细菌性阴道病中的乳酸菌及其噬菌体

• 批准号: 7231218
• 负责人: ARTHUR L REINGOLD
• 金额: $ 7.32万
• 依托单位: UNIVERSITY OF CALIFORNIA BERKELEY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-30 至 2009-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7231218
• 关键词: Address; Age; Area; Attention; Bacteria; Bacterial Vaginosis; Bacteriophage Typing; Bacteriophages; Behavior; Birth; Characteristics; Child; Childhood; Clinic; Cohort Studies; Collaborations; Communities; Condition; Cross-Sectional Studies; Cytolysis; Data; Development; Endometritis; Enrollment; Epidemiologic Studies; Epidemiology; Etiology; Fetal Growth Retardation; Follow-Up Studies; Foundations; Genomics; Goals; Herpes Simplex Infections; High Prevalence; Hormonal Change; Human Herpesvirus 2; Hydrogen Peroxide; India; Infant; Infection; Infection of amniotic sac and membranes; Intervention; Joints; Lactobacillus; Low Birth Weight Infant; Lytic; Maternal Mortality; Maternal and Child Health; Mediating; Menstrual cycle; Morbidity - disease rate; Mothers; Neonatal; Outcome; Participant; Play; Population; Postpartum Period; Premature Labor; Premature Rupture Fetal Membranes; Public Health; Range; Rate; Relative (related person); Research; Research Infrastructure; Research Personnel; Risk; Risk Factors; Role; Sexually Transmitted Diseases; Spermatocidal Agents; Staging; Subgroup; Testing; Time; Training; Vagina; Vaginal Douching; Virus; Woman; care seeking; cohort; cost; microorganism; mortality; neonate; prospective; reproductive; vaginal fluid; vaginal lactobacilli

DESCRIPTION (provided by applicant): The proposed study will investigate risk factors for bacterial vaginosis in and characterize Lactobacillus strains and Lactobacillus phages from women of reproductive age in Mysore India. Taking advantage of an existing prospective cohort study of Herpes Simplex 2 infection that has enrolled 750 women of reproductive age, we will enroll 200 women in an exploratory cross-sectional study to investigate the risk factors for bacterial vaginosis among reproductive age women in Mysore, India, and identify and characterize Lactobacillus and Lactobacillus phages among this population. In addition we will carry out a feasibility sub- study following 20 women bimonthly for three months to determine if we can isolate phages directly from women with intermediate vaginal flora, and determine if phage type distribution changes over time in vaginal fluids. This study, the first of its kind ever to be conducted in India, will lay the foundation to test the hypothesis that bacteriophage might be responsible for causing bacterial vaginosis. The aims of the study are to: 1) Collect information about risk factors and correlates of bacterial vaginosis among a population of reproductive-age women in Mysore, India; 2) Identify the Lactobacillus species found in vaginal fluids of a population of reproductive-age women in Mysore, India; 3) Analyze the distribution and types of phages isolated from vaginal Lactobacillus found in vaginal fluids of a population of reproductive-age women in Mysore, India; 4) Investigate the relative change, over time, in phage types found in the vaginal fluids of a small subgroup of women identified as having intermediate vaginal flora. The proposed research addresses the goals of MCHDR in the following ways: 1) It facilitates collaboration on maternal and children's health and contributes to a global understanding of the causes of morbidity and mortality in mothers and children. The research will facilitate the transfer of technical capacity and training to Indian researchers; build infrastructure in a setting where bacterial vaginosis has high public health importance because of its contribution to mortality and morbidity among neonates; focus much needed attention on a condition that has received minimal attention in the past; and provide an opportunity for both Indian and U.S. investigators to better understand the epidemiology, culturally specific risk factors, and role of phages in the etiology of bacterial vaginosis; 2) It addresses a leading cause of maternal, neonatal, and pediatric morbidity and mortality and India and; 3) It focuses on a "high priority" area for MCHDR: investigating "the risk factors for adverse birth outcomes including low birth weight and intrauterine growth retardation".

描述（由申请人提供）：拟议的研究将调查印度迈索尔育龄妇女的细菌性阴道病的危险因素并表征其乳酸菌菌株和乳酸菌噬菌体。利用现有的 2 型单纯疱疹感染前瞻性队列研究（已招募 750 名育龄妇女），我们将招募 200 名妇女参与一项探索性横断面研究，以调查印度迈索尔育龄妇女细菌性阴道病的危险因素，并鉴定和表征该群体中的乳杆菌和乳杆菌噬菌体。此外，我们将每两个月对 20 名女性进行一项为期三个月的可行性子研究，以确定我们是否可以直接从具有中间阴道菌群的女性中分离出噬菌体，并确定阴道液中噬菌体类型的分布是否随时间而变化。这项研究是印度首次进行的此类研究，将为检验噬菌体可能导致细菌性阴道病的假设奠定基础。该研究的目的是： 1) 收集印度迈索尔育龄妇女群体细菌性阴道病的危险因素和相关信息； 2) 鉴定印度迈索尔育龄妇女阴道液中发现的乳酸菌种类； 3) 分析从印度迈索尔育龄妇女阴道液中发现的阴道乳杆菌中分离出的噬菌体的分布和类型； 4) 研究一小部分被确定具有中间阴道菌群的女性阴道液中噬菌体类型随时间的相对变化。拟议的研究通过以下方式实现 MCHDR 的目标： 1) 它促进孕产妇和儿童健康方面的合作，并有助于全球了解孕产妇和儿童发病和死亡的原因。该研究将促进向印度研究人员转让技术能力和培训；在细菌性阴道病具有高度公共卫生重要性的环境中建设基础设施，因为它会导致新生儿死亡率和发病率；将急需的注意力集中在过去很少受到关注的情况上；并为印度和美国研究人员提供机会，更好地了解流行病学、文化特定风险因素以及噬菌体在细菌性阴道病病因学中的作用； 2) 它解决了印度孕产妇、新生儿和儿科发病率和死亡率的主要原因； 3) 重点关注 MCHDR 的“高度优先”领域：调查“不良出生结局的危险因素，包括低出生体重和宫内生长迟缓”。