Peptide hydrogels: A 3D in vitro model for liver toxicity.

肽水凝胶：肝毒性的 3D 体外模型。

• 批准号: 2905682
• 金额: --
• 依托单位: University of Manchester
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2905682
• 关键词: Peptide; hydrogels; 3D; vitro; model

Liver diseases are a significant medical burden, due to the lack of donors. There is a heavy global reliance on animal testing with a wide variety of animals used, and a major cause of cessation of drug development is secondary liver toxicity. The most common toxicity associated with drug withdrawals in the US and Europe are hepatic at 21%. This project exploits an engineered 3D culture system that maintains hepatocyte specific functions for liver toxicity testing using self-assembled peptide hydrogels that mimic the hepatic microenvironment. Optimal gels will be characterised for material physical and chemical properties, cell behaviour (using biochemical assays, immunocytochemistry/imaging and gene and protein expression), to determine the optimal gel system that can support the appropriate cells in long term culture, that can allow the cells to respond to toxins. This may then prove to be an appropriate in vitro 3D culture platform for liver toxicity testing.

由于缺乏捐助者，肝病是一个重大的医疗负担。全球对动物测试的严重依赖，使用了各种各样的动物，而停止药物开发的主要原因是肝脏毒性。在美国和欧洲，与取消药物有关的最常见毒性为21％。该项目利用了一种工程化的3D培养系统，该系统使用模仿肝微环境的自组装肽水凝胶维持肝细胞特异性功能，用于肝脏毒性测试。最佳凝胶将以物质和化学特性，细胞行为，使用生化测定，免疫细胞化学/成像以及基因和蛋白质表达来表征最佳凝胶，以确定可以在长期培养中支持适当细胞的最佳凝胶系统，从而使细胞可以允许细胞对毒素反应。然后，这可能被证明是用于肝毒性测试的合适体外3D培养平台。