EPR Sample-cell-Resonator Design and Construction

EPR 样品池谐振器设计与构造

• 批准号: 7272690
• 负责人: JAMES S HYDE
• 金额: $ 3.11万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-08-07 至 2009-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7272690
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DESCRIPTION (provided by applicant): This application is submitted in accordance with PA-02-057, Fogarty International Research Collaboration Award (FIRCA).The research will be done primarily in Poland, at the Jagiellonian University in Krakow in collaboration with Prof. Wojciech Froncisz, PhD, DSc, who is the Foreign Collaborator. It is an extension of NIH grant R01 EB001417, which is the Parent Grant. This proposal is device-design driven. The goal of the proposed work is to improve sensitivity in electron paramagnetic resonance (EPR) spectroscopy of aqueous fluid-phase samples. The proposal is timely because of recent advances in software for finite-element modeling of electromagnetic fields, computers with greatly improved computing speeds, and computer- controlled electric discharge machining (EDM). Computer-aided design will be used for resonators, sample cells and field modulation coils, followed by experimental evaluation. There are three Specific Aims, each of which involves the construction and evaluation in Poland of a sample-containing microwave resonator for EPR spectroscopy: (1) Loop-Gap Resonators (LGR) for Aqueous Samples. An LGR will be developed with ultra-high efficiency. (2) Uniform Field Cavity Resonators for Aqueous Samples. A Q-band uniform field cavity resonator will be developed for use with a novel sample-cell geometry and (3) Aqueous Sample Cell Clusters. A new Multipurpose cavity will be developed for use with layered flat cells. A theme of all three designs is use of EDM graphite, which has recently been proposed by us for EPR resonator construction. The P.I. serves as Director of The National Biomedical EPR Center, P41 EB00198Q, which is a Research Resource. Resonators developed under this FIRCA proposal will enhance the large number of Collaborative and Service Research projects carried out at the Resource. EPR spectroscopy is an important modality in biomedical research. Studies of short-lived radicals detected by spin trapping, of molecular structure using site-directed spin labeling, of biological or model membranes using spin probes, and of cell or tissue preparations are usually carried out in an aqueous environment. Work proposed here will improve the quality of data obtained in these experiments.

描述（由申请人提供）：该申请是根据Fogarty International Research Collakoration Award（FIRCA）根据PA-02-057提交的。该研究将主要在Krakow的Jagiellonian University在波兰与Wojciech Froncisz，Phd，Phd，Phd，DSC，Who是外国合作者的合作。它是NIH Grant R01 EB 001417的扩展，这是父母的赠款。该建议是设备设计驱动的。提出的工作的目的是提高水流体相样样品的电子顺磁共振（EPR）光谱的敏感性。该提案之所以及时，是因为电磁场有限元建模的软件，具有大大提高的计算速度的计算机以及计算机控制的电气放电加工（EDM）的有限元建模。计算机辅助设计将用于谐振器，样品单元和现场调制线圈，然后进行实验评估。有三个特定的目的，每个目标涉及在波兰进行EPR光谱的含样品微波谐振器的构造和评估：（1）用于水性样品的环路差异谐振器（LGR）。 LGR将以超高的效率开发。 （2）水性样品的均匀场腔谐振器。将开发一个Q波段均匀的场腔谐振器，用于与新型样品细胞几何形状和（3）水性样品细胞簇一起使用。将开发出一种新的多功能腔，以与分层的扁平细胞一起使用。这三种设计的主题是使用EDM石墨，最近我们提出了EPR共振构建。 P.I.是国家生物医学EPR中心的主任P41 EB 00198Q，这是一种研究资源。根据该FIRCA提案开发的共振器将增强在资源下进行的大量协作和服务研究项目。 EPR光谱是生物医学研究中的重要方式。通过自旋陷阱，使用位置定向的自旋标记，使用自旋探针的生物或模型膜的分子结构检测到的短寿命自由基的研究以及细胞或组织制剂的研究通常在水性环境中进行。这里提出的工作将提高这些实验中获得的数据质量。