Identification and functional analysis of renal stem cells

肾干细胞的鉴定及功能分析

基本信息

批准号：
7319589
负责人：
ROSEMARY V SAMPOGNA
金额：
$ 13万
依托单位：
COLUMBIA UNIVERSITY HEALTH SCIENCES
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-08-09 至 2012-07-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7319589
关键词：
Acute Kidney Failure Acute Kidney Tubular Necrosis Address Adult Analgesics Animal Model Antibodies Area Award Behavior Biochemistry Biological Biopsy Cell Cycle Cells Cellular biology Clinical Condition Cues Data Depth Detection Development Diagnostic Disease Doctor of Philosophy Embryo End stage renal failure Environment Epithelial Equilibrium Evolution Fellowship Genetic Transcription Goals Hematopoietic stem cells Human Hypoxia Injury Internal Medicine Invasive Investigation Kidney Kidney Diseases Kidney Papilla Kidney Part Label Laboratories Lead Life Localized Mediating Medical Mentored Clinical Scientist Development Award (K08)Methodology Methods Microscopy Molecular Morbidity - disease rate Mus Nephrology Numbers Organ Papillary Phenotype Physiologic pulse Population Prevalence Process Proliferating Property Protein Overexpression Public Health Pulse taking Rattus Regulation Renal function Reperfusion Injury Research Personnel Residencies Role Rosemary Scientist Sickle Cell Anemia Signal Transduction Skin Sorting - Cell Movement Source Stem cells Stimulus Structure Techniques Therapeutic Thinking Time Tissues Training Transcript Ureteral obstruction base body system cell behavior cell injury human tissue improved in vivo insight interest kidney cell mortality multipotent cell nephrogenesis programs relating to nervous system repaired research study response restoration skills tissue culture tissue regeneration tongue papilla tool transcription factor

项目摘要

DESCRIPTION (provided by applicant): This is an application for a Mentored Clinical Scientist Development Award (K08) entitled "Identification and Functional Analysis of Renal Stem Cells." The candidate holds MD and PhD degrees, has completed a residency in Internal Medicine and a fellowship in Nephrology. During this award, the candidate will acquire the background and laboratory skills required to further develop as a clinical scientist in the areas of renal disease and kidney development. The goals of the proposal are: (1) To identify a specific cell marker that is unique to label retaining cells in the kidney papilla and can be used to enrich and track this population; (2) To delineate the function of kidney label retaining cells in vivo before and after injury; and (3) To identify and delineate the function of regulatory molecules in kidney label retaining cells that mediate injury in acute tubular necrosis and obstructive nephropathy. The proposed studies utilize techniques in tissue culture, microscopy, molecular and cell biology, and this program of research and study will strengthen and advance the training of the candidate in these areas. The candidate proposes an integrated program of study and research directed toward improved understanding of reparative processes and, more specifically, the response of renal progenitor cells to injury. Indeed, an ever-increasing number of investigations demonstrate that tissue regeneration occurs throughout adult life, often in organ systems thought to be irreparable. Such studies point to the post-injury restoration of biological structure and function via cell-replacement strategies. The need for a deep understanding of stem cell behavior is central to confronting these issues. Relevance of this proposal to public health: the prevalence of acute renal failure in the hospitalized setting remains high and multiplies in the setting of increased co-morbid conditions. Moreover, diseases initially involving the renal papilla such as analgesic nephropathy and sickle cell anemia often lead to more global renal injury and can lead to end stage renal disease. Only by understanding the molecular signaling that underlies the "sensing" of renal injury as well as the initiation of repair, will we be able to initiate application of this new methodology to long-standing medical problems in nephrology.

描述（由申请人提供）：这是一份题为“肾干细胞的鉴定和功能分析”的指导临床科学家发展奖（K08）的申请。该候选人拥有医学博士和博士学位，已完成内科住院医师培训和肾脏病学专科培训。在此奖项期间，候选人将获得进一步发展成为肾脏疾病和肾脏发育领域的临床科学家所需的背景和实验室技能。该提案的目标是：（1）确定一种特定的细胞标记物，该标记物对于标记肾乳头中的保留细胞是独特的，并且可用于富集和跟踪该群体； (2) 描绘损伤前后体内肾标记保留细胞的功能； (3) 鉴定和描述肾标记保留细胞中介导急性肾小管坏死和梗阻性肾病损伤的调节分子的功能。拟议的研究利用组织培养、显微镜、分子和细胞生物学技术，该研究计划将加强和推进候选人在这些领域的培训。候选人提出了一项综合研究计划，旨在提高对修复过程的理解，更具体地说，是肾祖细胞对损伤的反应。事实上，越来越多的研究表明，组织再生发生在整个成年过程中，通常发生在被认为无法修复的器官系统中。此类研究指出，通过细胞替代策略可以在损伤后恢复生物结构和功能。深入了解干细胞行为的需要是应对这些问题的核心。该提案与公共卫生的相关性：住院环境中急性肾衰竭的患病率仍然很高，并且在合并症增加的情况下会成倍增加。此外，最初涉及肾乳头的疾病，如镇痛性肾病和镰状细胞性贫血，通常会导致更严重的整体肾损伤，并可能导致终末期肾病。只有了解肾损伤“感知”以及修复启动背后的分子信号传导，我们才能将这种新方法应用于肾脏病学中长期存在的医学问题。