New genetic approaches analyzing Borrelia burgdorferi

分析伯氏疏螺旋体的新遗传学方法

• 批准号: 6936194
• 负责人: Jon Scott Blevins
• 金额: $ 4.99万
• 依托单位: UT SOUTHWESTERN MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2008-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6936194
• 关键词: Borrelia; Lyme disease; bacterial genetics; gene mutation; genetic manipulation; laboratory mouse; laboratory rat; postdoctoral investigator; virulence

DESCRIPTION (provided by applicant): The study of Lyme disease has been hampered by the intractability of the etiological agent, Borrelia burgdorferi (Bb), to be genetically manipulated. This project will explore new avenues to improve the efficiency of genetically manipulating Bb; such developments will be applied towards understanding the role(s) of selected genes in virulence expression and/or colonization of hosts (ticks and mammals). The Specific Aims are: (1) To develop a novel insertion mutagenesis system (termed "RMEM") that will minimize the undesirable spontaneous loss of virulence that typically accompanies the genetic manipulation of Bb and (2) To apply the newly designed RMEM mutagenesis system to inactivate genes in a key regulatory network (e.g., rpoN-rpoS) and those involved in a stringent-like response (e.g. ndk, and spoT) to clarify their role(s) in regulating virulence expression and/or host colonization by Bb. This proposal thus seeks to contribute major new advances to borrelial genetics and to use those developments to examine potentially novel aspects of Bb virulence expression.

描述（由申请人提供）：莱姆病的研究因病原伯氏疏螺旋体（Bb）难以进行基因操纵而受到阻碍。该项目将探索提高基因操纵Bb效率的新途径；这些进展将用于了解选定基因在毒力表达和/或宿主（蜱和哺乳动物）定植中的作用。具体目标是：(1) 开发一种新型插入诱变系统（称为“RMEM”），该系统将最大限度地减少通常伴随 Bb 基因操作的不良自发毒力丧失；(2) 应用新设计的 RMEM 诱变系统使关键调控网络（例如 rpoN-rpoS）中的基因以及参与严格样反应（例如 ndk 和 spoT）的基因失活阐明它们在调节 Bb 毒力表达和/或宿主定植中的作用。因此，该提案旨在为疏螺旋体遗传学做出重大新进展，并利用这些进展来检查疏螺旋体毒力表达的潜在新颖方面。