Biacore T100

比亚科T100

• 批准号: 7215088
• 负责人: Charles Joel Arntzen
• 金额: $ 36.61万
• 依托单位: ARIZONA STATE UNIVERSITY-TEMPE CAMPUS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-03-01 至 2009-02-28
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7215088
• 关键词: Advisory Committees; Agriculture; Arizona; Automation; Award; Binding; Biological; Biology; Chemistry; Complement; Computer software; Conflict (Psychology); Core Facility; DNA laboratory; Data; Discipline; Disease; Ecology; Electronics; Engineering; Faculty; Fees; Grant; Growth; Hour; Housing; Human Resources; Institutes; Malignant Neoplasms; Medicine; Minor; Neurologic; Physics; Postdoctoral Fellow; Protein Chemistry; Public Health; Recruitment Activity; Research; Schedule; Science; Students; Surface Plasmon Resonance; Systems Analysis; Technology; Training; Universities; Wages; biodefense; day; experience; improved; instrument; vaccinology

DESCRIPTION (provided by applicant): This award will support the acquisition of a Biacore T100 molecular interaction analysis system at Arizona State University. The T100 instrument, which utilizes surface plasmon resonance (SPR) technology, will complement the two SPR instruments currently in use on the campus, a Biacore X and a FlexChip, neither of which has the capabilities or capacity to satisfy the growing needs for characterizing molecular interactions at ASU. Growth at ASU has included a net increase of 125 tenured and tenure-track faculty during the past two years with a corresponding growth in scientific research. The Biodesign Institute at Arizona State University is an exciting venture in merging of scientific disciplines, with faculty from the fields of biology, chemistry, physics, medicine, agriculture, environmental science, electronics, materials science, engineering and computing. The Biacore T100 offers the automation, improved software, and sensitivity that is required to take analysis further than is possible with either of the two instruments currently available on campus. The instrument will be housed at The Biodesign Institute, where it will be readily accessible to all PIs whose labs are located in the building, and is in close proximity to PIs located in other buildings. We plan to recruit a postdoctoral fellow with SPR experience to be the manager of the facility. The salary support for the manager will be initially shared by the major users of the instrument, but will eventually be paid from the usage fees generated by the instrument. ASU has several recoverable core facilities on campus which have operated successfully for 5-10 years (such as DNA Laboratory, Protein Chemistry Lab, EM Lab). Scheduling of the instrument will be electronic, and conflicts will be resolved by an advisory committee consisting of the PI of the grant, two major users, and one minor user. Approximately 15% of the available hours will be reserved for new and outside users. We currently have sent 6 graduate students to Biacore for training, and those labs have been able to generate preliminary data since the training. We plan to send additional personnel to training when the instrument has been delivered on campus. Though we have some access to the Biacore X, it is heavily utilized already (greater than 50% by two major users) and cannot fill the needs of our expanding faculty. Preliminary data has also been generated on the FlexChip located on campus, but further analysis requires the greater sensitivity available with a Biocore T100. Our needs have far outgrown the currently available instrument accessibility, and the purchase of the T100 will greatly advance the research schedules. Public Health Relevance: Our herein described projects encompass vaccinology, cancer and disease treatments, neurological therapies, and biodefense; an understanding of how biological molecules interact is a prerequisite to successful pursuit of answers to the relevant problems faced each day by Public Health officials globally.

描述（由申请人提供）：该奖项将支持在亚利桑那州立大学购买 Biacore T100 分子相互作用分析系统。 T100 仪器采用表面等离子共振 (SPR) 技术，将补充校园内目前使用的两种 SPR 仪器（Biacore X 和 FlexChip），这两种仪器都没有能力或容量来满足表征分子不断增长的需求。在亚利桑那州立大学的互动。亚利桑那州立大学的增长包括在过去两年中净增加了 125 名终身教授和终身教授，科学研究也相应增长。亚利桑那州立大学生物设计研究所是一个令人兴奋的科学学科融合项目，其教师来自生物学、化学、物理、医学、农业、环境科学、电子、材料科学、工程和计算机领域。 Biacore T100 提供了自动化、改进的软件和灵敏度，比校园内现有的两种仪器中的任何一种都可以进行更深入的分析。该仪器将安置在生物设计研究所，实验室位于该大楼内的所有 PI 都可以轻松使用该仪器，并且与其他大楼内的 PI 非常接近。我们计划招聘一名具有SPR经验的博士后担任该设施的经理。经理的薪资支持最初将由该工具的主要用户共享，但最终将从该工具产生的使用费中支付。 ASU 校园内拥有多个可恢复的核心设施，已成功运行 5-10 年（如 DNA 实验室、蛋白质化学实验室、EM 实验室）。该工具的安排将采用电子方式，冲突将由由资助项目的 PI、两名主要用户和一名次要用户组成的咨询委员会解决。大约 15% 的可用时间将保留给新用户和外部用户。目前我们已经派出了6名研究生到Biacore进行培训，培训结束后这些实验室已经能够产生初步数据。当仪器交付到校园后，我们计划派遣更多人员参加培训。尽管我们可以使用 Biacore X，但它已经被大量使用（两个主要用户的使用率超过 50%），无法满足我们不断扩大的教师队伍的需求。校园内的 FlexChip 也生成了初步数据，但进一步分析需要 Biocore T100 提供更高的灵敏度。我们的需求已经远远超出了当前可用仪器的可及性，购买 T100 将大大推进研究进度。公共卫生相关性：我们在此描述的项目包括疫苗学、癌症和疾病治疗、神经治疗和生物防御；了解生物分子如何相互作用是成功寻求全球公共卫生官员每天面临的相关问题答案的先决条件。