Hopkins Bayview Confocal Imaging System

霍普金斯 Bayview 共焦成像系统

• 批准号: 7212879
• 负责人: LANDON S KING
• 金额: $ 43.3万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7212879
• 关键词: Accounting; Aging; Allergic Reaction; Asthma; Award; Baltimore; Blood Vessels; Calcium; Cardiac; Cessation of life; Clinical Immunology; Computer software; Disease; Drug abuse; Elderly; Faculty; Funding; Future; Healthcare Systems; Hospitals; Housing; Hypersensitivity; Image; Institutes; Intramural Research Program; Laboratory Research; Life; Lipids; Lung; Medical center; Medicine; Methods; Microscope; Minor; Molecular; Numbers; Organelles; Phosphotransferases; Photons; Publishing; Request for Proposals; Research; Research Personnel; Resources; Sampling; System; Three-Dimensional Imaging; Time; United States; Universities; Urban Hospitals; base; cellular imaging; foot; instrument; intracellular protein transport; optical imaging; protein localization location; protein protein interaction

DESCRIPTION (provided by applicant): This proposal requests funds for the purchase of a Zeiss LSM 510 confocal microscope system that will be dedicated to the cellular imaging needs of five NIH-funded Major Users located on the Bayview campus of the Johns Hopkins University. This campus contains the Johns Hopkins Bayview Medical Center Hospital, the Hopkins Geriatric Center and Asthma and Allergy Center, the renovated Baltimore City Hospital containing 60,000 square feet of renovated research laboratories, and three buildings housing the intramural research programs of the National Institute on Aging (NIA) and National Institutes in Drug Abuse (NIDA). The Hopkins Bayview faculty account for one-third of the total research dollars awarded to the Department of Medicine (~$50 million dollars in FY 2005), and collectively, has published more than 3,700 articles during the period of 2000-2005. There is a dire need for advanced optical imaging resources on this campus, yet there is no multi- user facility here. The closest such facility is 4.2 miles away at the Hopkins East Baltimore campus. Although a shuttle bus connects the two campuses, transferring samples downtown is cumbersome and impractical for live cell imaging, the focus of a number of investigators in this application. Although there are several confocal microscopes on campus that belong to the NIA and NIDA intramural research programs, there is no unencumbered mechanism for Hopkins Bayview investigators to access these instruments. The instrument will initially be dedicated to the needs of the five major users, whose research focuses on the molecular and cellular understanding of clinical immunology and allergic reactions as well as vascular, cardiac, pulmonary, and rheumatoid diseases. Requests for imaging from a minor user group that currently includes an additional 14 NIH-funded investigators on the Bayview campus will be accommodated as time on the instrument allows. The combined group has critical needs for (1) co-localization of proteins and/or lipids to each other and to sub- cellular organelles; (2) FRET-based methods for characterizing protein-protein interactions, intracellular kinase activity, pH, and calcium; (3) multi-fluorescent live cell imaging; and (4) multi-fluorescent 3-D imaging. The confocal imaging system requested not only meets these needs, it is readily capable of accommodating the future addition of spectral deconvolution hardware and software (META) as well as 2-photon capabilities. The instrument will serve as the cornerstone for an imaging facility to meet the current and future needs of investigators at Hopkins Bayview. RELEVANCE: Research of the combined user groups is focused on the molecular and cellular understanding of clinical immunology and allergic reactions, as well as vascular, cardiac, pulmonary, and rheumatoid diseases. Collectively, these diseases represent a significant burden on the health care system and account for a large fraction of deaths in the United States every year.

描述（由申请人提供）：该提案请求资金购买 Zeiss LSM 510 共焦显微镜系统，该系统将专门满足位于约翰·霍普金斯大学 Bayview 校区的 5 个由 NIH 资助的主要用户的细胞成像需求。该校区包括约翰·霍普金斯湾景医疗中心医院、霍普金斯老年中心和哮喘和过敏中心、翻新后的巴尔的摩市医院（包含 60,000 平方英尺的翻新研究实验室）以及三栋容纳国家老龄化研究所校内研究项目的建筑（ NIA）和国家药物滥用研究所（NIDA）。霍普金斯湾景学院的教员占医学系研究经费总额的三分之一（2005 财年约为 5,000 万美元），2000 年至 2005 年期间总共发表了 3,700 多篇文章。该园区迫切需要先进的光学成像资源，但这里没有多用户设施。最近的此类设施位于 4.2 英里外的霍普金斯东巴尔的摩校区。尽管穿梭巴士连接两个校区，但在市中心转移样本对于活细胞成像来说很麻烦且不切实际，而活细胞成像是许多研究人员在该应用中关注的焦点。尽管校园内有几台属于 NIA 和 NIDA 校内研究项目的共焦显微镜，但霍普金斯湾景分校的研究人员没有无障碍的机制来访问这些仪器。该仪器最初将致力于满足五个主要用户的需求，这些用户的研究重点是临床免疫学和过敏反应以及血管、心脏、肺和类风湿疾病的分子和细胞理解。目前，Bayview 校区还有 14 名 NIH 资助的研究人员，其中一个小用户组提出的成像请求将在仪器使用时间允许的情况下得到满足。联合小组对以下方面具有关键需求：（1）蛋白质和/或脂质彼此以及亚细胞器的共定位； (2) 基于 FRET 的方法，用于表征蛋白质-蛋白质相互作用、细胞内激酶活性、pH 和钙； (3)多重荧光活细胞成像； (4) 多荧光 3-D 成像。所需的共焦成像系统不仅满足这些需求，而且还能够轻松适应未来添加的光谱反卷积硬件和软件 (META) 以及 2 光子功能。该仪器将作为成像设施的基石，以满足霍普金斯湾景研究人员当前和未来的需求。相关性：联合用户组的研究重点是对临床免疫学和过敏反应以及血管、心脏、肺和类风湿疾病的分子和细胞理解。总的来说，这些疾病给医疗保健系统带来了沉重负担，并且每年在美国造成的死亡人数中占很大一部分。