Hopkins Bayview Confocal Imaging System

霍普金斯 Bayview 共焦成像系统

• 批准号: 7212879
• 负责人: LANDON S KING
• 金额: $ 43.3万
• 依托单位: JOHNS HOPKINS UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7212879
• 关键词: Accounting; Aging; Allergic Reaction; Asthma; Award; Baltimore; Blood Vessels; Calcium; Cardiac; Cessation of life; Clinical Immunology; Computer software; Disease; Drug abuse; Elderly; Faculty; Funding; Future; Healthcare Systems; Hospitals; Housing; Hypersensitivity; Image; Institutes; Intramural Research Program; Laboratory Research; Life; Lipids; Lung; Medical center; Medicine; Methods; Microscope; Minor; Molecular; Numbers; Organelles; Phosphotransferases; Photons; Publishing; Request for Proposals; Research; Research Personnel; Resources; Sampling; System; Three-Dimensional Imaging; Time; United States; Universities; Urban Hospitals; base; cellular imaging; foot; instrument; intracellular protein transport; optical imaging; protein localization location; protein protein interaction

DESCRIPTION (provided by applicant): This proposal requests funds for the purchase of a Zeiss LSM 510 confocal microscope system that will be dedicated to the cellular imaging needs of five NIH-funded Major Users located on the Bayview campus of the Johns Hopkins University. This campus contains the Johns Hopkins Bayview Medical Center Hospital, the Hopkins Geriatric Center and Asthma and Allergy Center, the renovated Baltimore City Hospital containing 60,000 square feet of renovated research laboratories, and three buildings housing the intramural research programs of the National Institute on Aging (NIA) and National Institutes in Drug Abuse (NIDA). The Hopkins Bayview faculty account for one-third of the total research dollars awarded to the Department of Medicine (~$50 million dollars in FY 2005), and collectively, has published more than 3,700 articles during the period of 2000-2005. There is a dire need for advanced optical imaging resources on this campus, yet there is no multi- user facility here. The closest such facility is 4.2 miles away at the Hopkins East Baltimore campus. Although a shuttle bus connects the two campuses, transferring samples downtown is cumbersome and impractical for live cell imaging, the focus of a number of investigators in this application. Although there are several confocal microscopes on campus that belong to the NIA and NIDA intramural research programs, there is no unencumbered mechanism for Hopkins Bayview investigators to access these instruments. The instrument will initially be dedicated to the needs of the five major users, whose research focuses on the molecular and cellular understanding of clinical immunology and allergic reactions as well as vascular, cardiac, pulmonary, and rheumatoid diseases. Requests for imaging from a minor user group that currently includes an additional 14 NIH-funded investigators on the Bayview campus will be accommodated as time on the instrument allows. The combined group has critical needs for (1) co-localization of proteins and/or lipids to each other and to sub- cellular organelles; (2) FRET-based methods for characterizing protein-protein interactions, intracellular kinase activity, pH, and calcium; (3) multi-fluorescent live cell imaging; and (4) multi-fluorescent 3-D imaging. The confocal imaging system requested not only meets these needs, it is readily capable of accommodating the future addition of spectral deconvolution hardware and software (META) as well as 2-photon capabilities. The instrument will serve as the cornerstone for an imaging facility to meet the current and future needs of investigators at Hopkins Bayview. RELEVANCE: Research of the combined user groups is focused on the molecular and cellular understanding of clinical immunology and allergic reactions, as well as vascular, cardiac, pulmonary, and rheumatoid diseases. Collectively, these diseases represent a significant burden on the health care system and account for a large fraction of deaths in the United States every year.

描述（由申请人提供）：该提案要求购买Zeiss LSM 510共聚焦显微镜系统的资金，该系统将专门针对约翰·霍普金斯大学Bayview校园的五个基金资助的主要用户的蜂窝成像需求。该校园里有约翰·霍普金斯湾医学中心医院，霍普金斯老年中心和哮喘和过敏中心，翻新的巴尔的摩市医院，其中包含60,000平方英尺的翻新研究实验室，以及三座建筑物，其中包含了国家研究所（NIA）和国家研究所（NIA）和国家毒品虐待（NIDA）的国家研究所内研究所的研究计划。霍普金斯湾景的教职员工占医学院授予的总研究资金的三分之一（2005财年为5000万美元），并且在2000 - 2005年期间已发表了3,700多篇文章。在这个校园里，需要高级光学成像资源，但这里没有多用户设施。在霍普金斯东巴尔的摩校园，最接近的设施位于4.2英里。尽管班车连接了这两个校园，但在现场成像中转移样本却很麻烦且不切实际，这是该应用程序中许多调查人员的重点。尽管校园里有几个共聚焦显微镜属于NIA和NIDA壁内研究计划，但霍普金斯Bayview调查人员没有访问这些工具的无限机制。该仪器最初将致力于五个主要使用者的需求，他们的研究重点是对临床免疫学和过敏反应以及血管，心脏，肺和类风湿病的分子和细胞理解。次要用户组的成像请求目前包括在Bayview校园内额外的14个由NIH资助的调查员，随着乐器上的时间允许的时间。组合组对（1）蛋白质和/或脂质共定位以及彼此的脂质以及亚细胞细胞器的关键需求； （2）基于FRET的方法，用于表征蛋白质 - 蛋白质相互作用，细胞内激酶活性，pH和钙； （3）多荧光活细胞成像； （4）多荧光3-D成像。共聚焦成像系统不仅可以满足这些需求，而且还可以容易地适应光谱反卷积硬件和软件（META）以及2光量功能的未来。该乐器将作为成像设施的基石，以满足霍普金斯湾观察人员当前和未来的需求。相关性：联合用户组的研究集中于对临床免疫学和过敏反应的分子和细胞理解，以及血管，心脏，肺和类风湿病。总的来说，这些疾病代表了医疗保健系统的重大负担，每年在美国占死亡人数很大。