A Time Domain In Vivo Optical Molecular Imaging Scanner for Small Animals

小动物体内时域光学分子成像扫描仪

• 批准号: 7220686
• 负责人: David J Hall
• 金额: $ 23.27万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-06-07 至 2009-06-06
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7220686
• 关键词: Affinity; Animals; Biochemistry; Blood flow; Chemistry; Development; Diagnosis; Disease model; Exhibits; Generations; Green Fluorescent Proteins; Human; Image; Imaging Device; Laboratories; Ligands; Malignant Neoplasms; Measurement; Measures; Molecular; Molecular Biology; Molecular Probes; Molecular Target; Optics; Permeability; Pharmacology; Physiological; Play; Positron-Emission Tomography; Process; Protocols documentation; Radiology Specialty; Research; Research Personnel; Rodent Diseases; Role; Time; Tissues; Translating; base; bench to bedside; cyanine dye 5; density; design; fluorescence imaging; human disease; in vivo; instrument; molecular imaging; optical imaging; programs; research study; tool; uptake

DESCRIPTION (provided by applicant): The specific aim of this proposal is to upgrade our single-wavelength optical imaging scanner for small animals. The upgrade will permit the scanner to operate at 4 wavelengths. This will provide new capabilities to enhance current research by permitting a variety of optical molecular probes to be imaged simultaneously. Currently we have a first generation of the eXplore Optix(tm) scanner, which only operates at 750 nm and is limited to imaging solely Cy7-based optical probes. The proposed upgrade will incorporate 3 additional wavelengths, namely the 470 nm, 650 nm, and 780 nm modules, which will permit time domain fluorescence imaging of GFP, Cy5, and Cy7.5 respectively. The ability to simultaneously measure two or more optical probes during the same experiment will permit the simultaneous measurement of two or more physiologic and/or molecular processes. Our small animal imaging laboratory also has PET and CT imaging devices, but the upgraded explore Optix(tm) scanner will be unique in its ability to control for permeability and/or flow at the same time that we are measuring biochemistry. The UCSD Molecular Imaging Program has many investigators in cancer, molecular biology, chemistry, pharmacology, and radiology. This proposal lists 12 projects that will benefit from the upgraded imager. The simultaneous measurement of multiple processes will provide a tool to answer a question that is central to most molecular image experiments: if a target tissue exhibits increased uptake, is the higher uptake due to increased delivery (blood flow or ligand permeability) or an increase in the affinity or density of the molecular target? At present, these measurements must be conducted in separate animals, which lowers the statistical power to quantify differences. Relevance: Molecular imaging of small animals will play a vital role in the development of imaging protocols for diagnosis and therapy. Properly designed optical imaging experiments permit the simultaneous measurement of multiple molecular processes. By simultaneously detecting optical molecular probes, the instrument can gather greater amounts of information from a single experiment. Consequently, the upgrade of our optical imager to multi-wavelength capability will promote the development towards long- term human applications of molecular imaging. Our long-term objective is to translate optical molecular probes from rodent disease models to the diagnosis and management of human disease. This upgrade will accelerate the path from bench to bedside.

描述（由申请人提供）：该提案的具体目的是升级我们的小动物单波长光学成像扫描仪。此次升级将使扫描仪能够在 4 个波长下运行。这将通过允许多种光学分子探针同时成像来提供新的能力来增强当前的研究。目前，我们拥有第一代 eXplore Optix(tm) 扫描仪，该扫描仪仅在 750 nm 下工作，并且仅限于仅对基于 Cy7 的光学探针进行成像。拟议的升级将包含 3 个附加波长，即 470 nm、650 nm 和 780 nm 模块，这将分别允许 GFP、Cy5 和 Cy7.5 的时域荧光成像。在同一实验期间同时测量两个或更多个光学探针的能力将允许同时测量两个或更多个生理和/或分子过程。我们的小动物成像实验室还拥有 PET 和 CT 成像设备，但升级后的 explore Optix(tm) 扫描仪的独特之处在于，它能够在测量生物化学的同时控制渗透性和/或流量。加州大学圣地亚哥分校分子成像项目拥有许多癌症、分子生物学、化学、药理学和放射学领域的研究人员。该提案列出了 12 个将受益于升级后的成像仪的项目。多个过程的同时测量将提供一个工具来回答大多数分子图像实验的核心问题：如果目标组织表现出吸收增加，那么吸收增加是由于输送增加（血流或配体渗透性）还是由于分子靶标的亲和力或密度？目前，这些测量必须在单独的动物中进行，这降低了量化差异的统计能力。相关性：小动物的分子成像将在诊断和治疗成像方案的开发中发挥至关重要的作用。正确设计的光学成像实验可以同时测量多个分子过程。通过同时检测光学分子探针，该仪器可以从单个实验中收集更多信息。因此，我们的光学成像仪升级到多波长能力将促进分子成像向人类长期应用的发展。我们的长期目标是将光学分子探针从啮齿动物疾病模型转化为人类疾病的诊断和管理。此次升级将加速从实验室到临床的进程。

项目成果

Analysis of the fluorescence temporal point-spread function in a turbid medium and its application to optical imaging.

混浊介质中荧光时间点扩散函数的分析及其在光学成像中的应用。

• DOI: 10.1117/1.3042271
• 发表时间: 2008
• 期刊: Journal of biomedical optics
• 影响因子: 3.5
• 作者: Han,Sung-Ho;Farshchi-Heydari,Salman;Hall,DavidJ
• 通讯作者: Hall,DavidJ