Microscopy Shared Resource

显微镜共享资源

• 批准号: 10400732
• 负责人: David J Hall
• 金额: $ 20.34万
• 依托单位: UNIVERSITY OF CALIFORNIA, SAN DIEGO
• 依托单位国家: 美国
• 财政年份: 1996
• 资助国家: 美国
• 起止时间: 1996-07-01 至 2024-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/10400732
• 关键词: 3-Dimensional; Animals; Behavior; California; Cancer Center; Cancer Center Support Grant; Cells; Collaborations; Complex; Conceptions; Consultations; Core Facility; Data Analyses; Disease Progression; Drug Delivery Systems; Electron Microscope; Equipment; Fluorescence; Fluorescence Resonance Energy Transfer; Fluorescent Probes; Fostering; Gene Expression Profile; Goals; Hour; Image; Imaging technology; Individual; Investigation; Leadership; Malignant Neoplasms; Methods; Microscopy; Molecular; Neurosciences; Organism; Pharmacotherapy; Phase-Contrast Microscopy; Photobleaching; Proteins; Reagent; Recovery; Research; Research Personnel; Resolution; Resource Sharing; Sampling; Services; System; Techniques; Training; Universities; anticancer research; cancer cell; cancer therapy; image processing; imaging approach; imaging system; improved; in vivo imaging; instrument; instrumentation; interest; member; non-invasive monitor; optical imaging; particle; photo switch; photoactivation; tool; trafficking; tumor progression

MICROSCOPY SHARED RESOURCE ABSTRACT Cancer research encompasses a broad range of investigations ranging from the behavior of cancer cells in the body, to the changes in cancer cells at the molecular level. This requires a spectrum of tools that enable imaging individual cells and their interactions, both at the level of the whole animal, as well as at the molecular level of molecules inside the cells. The Microscopy Shared Resource (MSR) provides training, access, support and expertise that enable Moores Cancer Center (MCC) members to successfully utilize basic and advanced microscopy techniques and instrumentation in their studies. The MSR facility is open 24 hours/7 days per week and provides access to a wide array of microscopy instrumentation, auxiliary equipment and reagents. The MSR is pivotal in providing investigators access and expertise to high-end instruments for their studies that they could not afford otherwise. These instruments include regular epi-fluorescence, bright field and phase contrast microscopy, confocal, super resolution, Forster Resonance Energy Transfer (FRET), total internal reflection fluorescence (TIRF), and deconvolution on fixed samples and live cells. The IVIS 200 imaging system can be used for 3-dimensional optical imaging and non-invasive monitoring of cancer progression, gene expression patterns, cell trafficking, drug delivery and treatment in living animals. Researchers can use super resolution fluorescence to examine interactions between proteins and other molecules using excitation/deactivation of photo-switchable fluorescent probes. The MSR staff provides highly qualified didactic and hands on training in basic microscopy principles in addition to complex techniques. By bringing together investigators with diverse interests and common goals, the MSR fosters a spirit of collaboration and cooperation that leads us closer to the ultimate goals of cancer treatment and cure.

显微镜共享资源摘要 癌症研究涵盖广泛的研究，从癌细胞在体内的行为 身体，到癌细胞在分子水平上的变化。这需要一系列工具来实现 在整个动物水平以及分子水平上对单个细胞及其相互作用进行成像 细胞内的分子水平。显微镜共享资源 (MSR) 提供培训、访问和支持 和专业知识，使摩尔斯癌症中心 (MCC) 成员能够成功利用基础和高级 他们研究中的显微镜技术和仪器。 MSR 设施每周 7 天、24 小时开放 并提供各种显微镜仪器、辅助设备和试剂。这 MSR 在为研究人员提供研究所需的高端仪器和专业知识方面发挥着关键作用 否则他们负担不起。这些仪器包括常规落射荧光、明场和相位 对比显微镜、共焦、超分辨率、福斯特共振能量转移 (FRET)、全内部 反射荧光 (TIRF)，以及固定样品和活细胞的解卷积。 IVIS 200 成像 系统可用于3维光学成像和癌症进展的无创监测， 活体动物的基因表达模式、细胞运输、药物输送和治疗。研究人员可以使用 超分辨率荧光检查蛋白质和其他分子之间的相互作用 光可切换荧光探针的激发/失活。 MSR 员工提供高素质的教学 除了复杂的技术之外，还进行基本显微镜原理的实践培训。通过汇集 MSR 培养了具有不同兴趣和共同目标的研究人员的合作精神 合作使我们更接近癌症治疗和治愈的最终目标。