QSTAR Elite Quadrupole-o-TOF LC/MS/MS System

QSTAR Elite 四极杆-o-TOF LC/MS/MS 系统

• 批准号: 7389246
• 负责人: Bradford Wayne Gibson
• 金额: $ 46.56万
• 依托单位: BUCK INSTITUTE FOR RESEARCH ON AGING
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-20 至 2008-09-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7389246
• 关键词: Aging; Alzheimer' s Disease; Arts; Biological Markers; Biology of Aging; Communicable Diseases; Complex; Complex Mixtures; Computer software; Disease; Experimental Designs; Funding; Generations; Genital system; Grant; Health; Human; Huntington Disease; Institutes; Institution; Ions; Iowa; Liquid Chromatography; Lung diseases; Malignant Neoplasms; Mass Spectrum Analysis; Meningeal Tuberculosis; National Center for Research Resources; Neurodegenerative Disorders; Numbers; Otitis Media; Parkinson Disease; Peptides; Phosphorylation; Post-Translational Protein Processing; Protein Array; Proteins; Proteomics; Range; Rate; Relative (related person); Research Personnel; Stroke; System; Time; Tularemia; Ulcer; United States National Institutes of Health; Universities; age related; improved; instrument; instrumentation; ion source; malignant breast neoplasm; mass spectrometer; new technology; transmission process

DESCRIPTION (provided by applicant): This NCRR shared instrumentation proposal seeks funding for the purchase of an Applied Biosystems/Sciex new 5th generation QSTAR Elite(r) quadrupole orthogonal time-of-flight mass spectrometer and associated Tempo (Eksigent) nanocapillary multidimensional liquid chromatography (MDLC) station. To complete this integrated LC MS/MS system, we are also asking for a nanospray ion source, as well as ProteinPilot 2.0 and MarkerView software packages. In brief, we propose to operate this integrated LC MS/MS almost exclusively for on-line separation and analysis of complex peptide (and protein) mixtures as part of large group of 24 NIH funded grants from 15 investigators who have significant needs in state-of-the-art proteomics. The most critical features of this new instrumentation will be to greatly improve on the number of tandem mass spectra that can be acquired (up to 7 MS/MS spectra/sec), overall sensitivity (low attomole), dynamic range (4 logs), mass range (5-40,000 m/z), collision energetics, ion transmission, and quantitation software features. The unique capabilities of this instrumentation will be directed at a large array of protein and proteomic applications where mass spectrometry is used for protein/peptide identification in complex mixtures, relative quantitation, as well as the determination of biologically important enzymatic and non-enzymatic posttranslational modifications, including protein phosphorylation, oxidative damage and nitrosylation, among others. Overall, the acquisition of this instrument will greatly improve the throughput, experimental design, and discovery rate of 16 projects that are examining key issues of human health, including aging, neurodegenerative disease, cancer, biomarker discovery, stroke and infectious diseases. The relevance of this new instrumentation to human health is significant, as 13 independent researchers at two institutions (Buck Institute and UCSF) will use this new technology to improve their ongoing studies in the basic biology of aging and age-related diseases, including Huntington's and Alzheimer's disease, Parkinson's disease, breast cancer, and stroke. In addition, a smaller group of four investigators at the Buck Institute, UCLA and University of Iowa, that are investigating pathogenic mechanisms in several human infectious diseases including tuberculosis, meningitis, genital ulcers, respiratory disease, tularemia, and otitis media.

描述（由申请人提供）：此 NCRR 共享仪器提案寻求资助购买 Applied Biosystems/Sciex 新型第五代 QSTAR Elite(r) 四极杆正交飞行时间质谱仪和相关的 Tempo (Eksigent) 纳米毛细管多维液相色谱仪（MDLC）站。为了完成这个集成的 LC MS/MS 系统，我们还需要纳喷雾离子源以及 ProteinPilot 2.0 和 MarkerView 软件包。简而言之，我们建议操作这种集成的 LC MS/MS 几乎专门用于复杂肽（和蛋白质）混合物的在线分离和分析，作为 24 名 NIH 资助的大型团体的一部分，该团体由 15 名研究人员资助，这些研究人员在国家/地区有重大需求 -最先进的蛋白质组学。这种新仪器最关键的特点是大大提高可采集的串联质谱数量（高达 7 MS/MS 谱图/秒）、整体灵敏度（低阿托摩尔）、动态范围（4 个对数）、质量范围 (5-40,000 m/z)、碰撞能量学、离子传输和定量软件功能。该仪器的独特功能将针对大量蛋白质和蛋白质组学应用，其中质谱法用于复杂混合物中的蛋白质/肽鉴定、相对定量以及生物学上重要的酶促和非酶促翻译后修饰的测定，包括蛋白质磷酸化、氧化损伤和亚硝基化等。总体而言，收购该仪器将极大提高16个项目的通量、实验设计和发现率，这些项目正在研究人类健康的关键问题，包括衰老、神经退行性疾病、癌症、生物标志物发现、中风和传染病。 这种新仪器与人类健康的相关性意义重大，因为两个机构（巴克研究所和加州大学旧金山分校）的 13 名独立研究人员将使用这项新技术来改进他们在衰老和与年龄相关的疾病（包括亨廷顿舞蹈症和老年痴呆症）的基础生物学方面正在进行的研究。阿尔茨海默病、帕金森病、乳腺癌和中风。此外，巴克研究所、加州大学洛杉矶分校和爱荷华大学的四名研究人员组成的小组正在研究几种人类传染病的致病机制，包括结核病、脑膜炎、生殖器溃疡、呼吸道疾病、兔热病和中耳炎。

项目成果

ScanRanker: Quality assessment of tandem mass spectra via sequence tagging.

• DOI: 10.1021/pr200118r
• 发表时间: 2011-07-01
• 期刊: JOURNAL OF PROTEOME RESEARCH
• 影响因子: 4.4
• 作者: Ma, Ze-Qiang;Chambers, Matthew C.;Ham, Amy-Joan L.;Cheek, Kristin L.;Whitwell, Corbin W.;Aerni, Hans-Rudolf;Schilling, Birgit;Miller, Aaron W.;Caprioli, Richard M.;Tabb, David L.
• 通讯作者: Tabb, David L.