VisualSonics Vevo770 Ultrasound Biomicroscopy and Microinjection Core

VisualSonics Vevo770 超声生物显微镜和显微注射核心

• 批准号: 7388389
• 负责人: Bradley Barth Keller
• 金额: $ 37.78万
• 依托单位: CHILDREN'S HOSP PITTSBURGH/UPMC HLTH SYS
• 依托单位国家: 美国
• 财政年份: 2007
• 资助国家: 美国
• 起止时间: 2007-09-20 至 2008-09-19
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7388389
• 关键词: Adult; Animal Model; Animals; Blood Pressure; Blood Vessels; Breeding; Caliber; Cardiac; Cardiovascular system; Cells; Central Artery; Communities; Core Facility; Data; Data Set; Depth; Embryo; Frequencies; Functional Imaging; Funding; Genotype; Heart Rate; Image; Individual; Injection of therapeutic agent; Laboratories; Magnetic Resonance Imaging; Microinjections; Mus; Pediatric Hospitals; Pediatric Research; Phenotype; Protocols documentation; Rate; Rattus; Research; Research Personnel; Resolution; Resources; Rodent; S10 grant; Scanning; Site; System; Techniques; Time; Ultrasonographic Biomicroscopy; Ultrasonography; cost; human disease; instrumentation; molecular modeling; novel; research facility; two-dimensional

DESCRIPTION (provided by applicant): This Shared Instrumentation Grant (S10) requests funds to purchase a VisualSonics Vevo770 Ultrasound Biomicroscopy and Microinjection Core (UBMIC) for use by both established NIH-funded and young investigators in the new John G. Rangos Pediatric Research Building at the Children's Hospital of Pittsburgh, Lawrenceville Campus. With the rapid expansion of molecular models of human diseases in small animals (predominantly but not exclusively in mice) the ability to perform longitudinal, high-resolution, efficient functional imaging that is accurate, efficient, and affordable to investigators has become essential for most research protocols. Micro-CT and small bore MRI systems provide outstanding spatial resolution but cannot be operated to provide efficient high-throughput, cannot provide cardiovascular functional data, and do not currently assist in targeting microinjection. Due to the significant costs of rodent colonies and the time required to generate unique cross-bred genotypes, it is essential to acquire comprehensive data sets rather than individual functional parameters (such as heart rate or blood pressure alone) whenever feasible. The novel VisualSonics high-resolution ultrasound imaging system uses scanning frequencies up to 82.5 MHz to provide 2-dimensional images with an axial resolution to 30 <m, depths of 20 mm, and imaging rates up to 1000 frames per second. The Vevo770 can generate detailed cardiac functional data and vascular flow velocity data from the 3 mm diameter central arteries to 30 <m diameter intracranial and intra-placental vessels. The Vevo770 can also image the developing mouse embryo non-invasively starting at ED 6.5 and earlier using para vivo techniques. By combining high-resolution ultrasound imaging with a microinjection system, the Vevo770 supports the accurate and efficient delivery of cells and compounds to target sites in developing embryos and adult rodents. While our research facility currently includes a variety of small animal imaging resources within individual investigator laboratories, these current systems are not configured or available to meet increasing investigator imaging and micro-injection demands. Our proposal outlines the (1) scientific need; (2) technical support, administration, oversight; (3) representative investigator research applications; (4) institutional commitment; and (5) academic community benefit of our proposed VisualSonics Vevo770 UBMIC facility at the Children's Hospital of Pittsburgh. The UBMIC will be managed by a team with established expertise in high-resolution ultrasound imaging, cardiovascular phenotyping, and instrumentation from the embryo to adult in chick, mouse, and rat species with substantial institutional support. Our proposed VisualSonics Vevo770 Ultrasound Biomicroscopy and Microinjection Core (UBMIC) facility provides core users with high-resolution functional imaging and micro-injection capability in order to better develop, characterize, and treat human diseases within small animal models.

描述（由申请人提供）：此共享仪器补助金 (S10) 请求资金购买 VisualSonics Vevo770 超声生物显微镜和显微注射核心 (UBMIC)，供 NIH 资助的老牌研究人员和新 John G. Rangos 儿科研究大楼的年轻研究人员使用在匹兹堡儿童医院劳伦斯维尔校区。随着小动物（主要但不限于小鼠）人类疾病分子模型的快速扩展，进行准确、高效且研究人员负担得起的纵向、高分辨率、高效功能成像的能力已成为大多数研究的关键协议。微型CT和小口径MRI系统提供出色的空间分辨率，但无法操作以提供高效的高通量，无法提供心血管功能数据，并且目前不协助靶向显微注射。由于啮齿动物群体的巨大成本以及产生独特的杂交基因型所需的时间，因此在可行的情况下有必要获取全面的数据集而不是单独的功能参数（例如单独的心率或血压）。新颖的 VisualSonics 高分辨率超声成像系统使用高达 82.5 MHz 的扫描频率，提供轴向分辨率为 30 <m、深度为 20 mm 的二维图像，成像速率高达每秒 1000 帧。 Vevo770 可以生成从 3 毫米直径中央动脉到 30 米直径颅内和胎盘内血管的详细心脏功能数据和血管流速数据。 Vevo770 还可以使用体外技术对 ED 6.5 或更早的发育中的小鼠胚胎进行非侵入性成像。通过将高分辨率超声成像与显微注射系统相结合，Vevo770 支持将细胞和化合物准确有效地输送到发育中的胚胎和成年啮齿动物的目标部位。虽然我们的研究设施目前在各个研究人员实验室内包括各种小动物成像资源，但这些当前系统的配置或无法满足日益增长的研究人员成像和显微注射需求。我们的提案概述了 (1) 科学需求； (2) 技术支持、管理、监督； （三）代表性研究者的研究申请； （四）制度承诺； (5) 我们提议的位于匹兹堡儿童医院的 VisualSonics Vevo770 UBMIC 设施的学术界利益。 UBMIC 将由一个团队管理，该团队在高分辨率超声成像、心血管表型分析和小鸡、小鼠和大鼠物种从胚胎到成年的仪器方面拥有丰富的专业知识，并获得大量机构支持。我们提出的 VisualSonics Vevo770 超声生物显微镜和显微注射核心 (UBMIC) 设施为核心用户提供高分辨率功能成像和显微注射功能，以便更好地开发、表征和治疗小动物模型中的人类疾病。