MOEMS device for fluorescence spectroscopy of tissues and cells

用于组织和细胞荧光光谱分析的 MOEMS 装置

• 批准号: 6983764
• 负责人: Laura Marcu
• 金额: $ 9.9万
• 依托单位: CEDARS-SINAI MEDICAL CENTER
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2005-12-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6983764
• 关键词: MOEMS; device; fluorescence; spectroscopy; tissues

DESCRIPTION (provided by applicant): The overall project objectives are to (i) develop an Micro-Opto-Electro-Mechanical System (MOEMS) device for resolving and detecting fluorescence emission from biological tissues and cells; (ii) provide knowledge and strategies about how spectroscopic micro-devices can be integrated in existing diagnostic instruments; and (iii) enable advanced, fully integrated platforms for biochemical assays and minimally-invasive medical diagnosis and surgery. The device will have broad applicability including diagnosis, localization and staging of cancer and other diseases, monitoring of therapeutic effects, spectroscopy/image guided biopsy and surgery. Because fluorescence spectroscopy is very sensitive to changes in functional and structural properties of molecules and molecular complexes in biological systems, fluorescence spectroscopy plays a key role on a broad area of biological and medical applications. Advances proposed here, using micro-fabrication techniques, allow construction of integrated spectroscopy micro-devices capable of real-time fluorescence measurements from tissues and cells. This will enable advances in miniature multifunctional instrumentation and synergy of diagnostic technologies. For example, integrating such device in endoscopes, biopsy probes, and surgical/monitoring devices will provide simultaneously information about biochemical and functional changes at the investigated side, thus the diagnostic capability of these instruments will be enhanced without altering their function and basic form. We propose to develop a MOEMS device consisting of a micro-fabricated dispersive system and photomultiplier that will replace the conventional monochromator and detector in a fluorescence spectroscopy apparatus and allow both steady-state and time-resolved (lifetime) measurements. Specific aims include: (1) To design, micro-fabricate, integrate and test a micro-monochromator (MMC). This is to be a multi-chip system including a series of key elements (piezoelectrically actuated cantilever with diffraction grating, mirrors and lenses). (2) To design, microfabricate, and demonstrate functionability of a multichannel plate photomultiplier (MM-MCP-PMT). The results of this R21 application will be a basis of knowledge sufficient to permit continued integration of the proposed MMC and MM-MCP-PMT into a compact MOEMS device in a future R01 application. This work will provide information about the spectroscopic performance (spectral resolution, sensitivity) and limitations of the MMC; and develop design solutions for an optimal MM-MCP-PMT. The knowledge gained will be used to improve the MOEMS design and to investigate pathways for further miniaturization of the electronics associated with the spectroscopic device. The device will provide the initial kernel for development of fully integrated miniature fluorescence spectroscopy devices and fluorescence-guided surgical/diagnostic micro-systems. The device will widely be applied to various biomedical instruments that require wavelength differentiation and selection.

描述（由申请人提供）：项目的总体目标是（i）开发一种微光机电系统（MOEMS）装置，用于解析和检测生物组织和细胞的荧光发射； (ii) 提供有关如何将光谱微型设备集成到现有诊断仪器中的知识和策略； (iii) 为生化检测和微创医疗诊断和手术提供先进、完全集成的平台。该设备将具有广泛的适用性，包括癌症和其他疾病的诊断、定位和分期、治疗效果监测、光谱/图像引导活检和手术。由于荧光光谱对生物系统中分子和分子复合物的功能和结构特性的变化非常敏感，因此荧光光谱在生物和医学应用的广泛领域中发挥着关键作用。这里提出的进步，使用微制造技术，允许构建能够实时测量组织和细胞荧光的集成光谱微型设备。这将促进微型多功能仪器和诊断技术协同的进步。例如，将此类设备集成到内窥镜、活检探针和手术/监测设备中，将同时提供有关受检侧生化和功能变化的信息，从而在不改变其功能和基本形式的情况下增强这些仪器的诊断能力。我们建议开发一种由微制造色散系统和光电倍增器组成的 MOEMS 设备，该设备将取代荧光光谱仪中的传统单色仪和检测器，并允许稳态和时间分辨（寿命）测量。具体目标包括：（1）设计、微制造、集成和测试微型单色仪（MMC）。这是一个多芯片系统，包括一系列关键元件（带有衍射光栅、镜子和透镜的压电驱动悬臂）。 (2) 设计、微加工并演示多通道板光电倍增管 (MM-MCP-PMT) 的功能。该 R21 应用的结果将成为足以允许将提议的 MMC 和 MM-MCP-PMT 继续集成到未来 R01 应用中的紧凑型 MOEMS 设备中的知识基础。这项工作将提供有关 MMC 的光谱性能（光谱分辨率、灵敏度）和局限性的信息；并开发最佳 MM-MCP-PMT 的设计解决方案。获得的知识将用于改进 MOEMS 设计并研究与光谱设备相关的电子设备进一步小型化的途径。该设备将为开发完全集成的微型荧光光谱设备和荧光引导手术/诊断微系统提供初始内核。该装置将广泛应用于各种需要波长区分和选择的生物医学仪器中。