Chronic Wounds Point-of-care system for monitoring moisture, temperature, pH, Interleukin-6 and Uric Acid levels

慢性伤口护理点系统，用于监测湿度、温度、pH、白细胞介素 6 和尿酸水平

• 批准号: 2889512
• 金额: --
• 依托单位: Imperial College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2889512
• 关键词: Chronic; Wounds; Point; care; system

Chronic wounds are those that take longer than three months to heal or do not show improvements after prompt treatment, being more likely to become infected or colonised by bacteria. Currently, the most prevalent methods for managing chronic wounds are visual assessment and diagnostic dressing, which have significant financial impacts on the healthcare sector. Chronic wounds impact 5.7 million people in the US and cost $20 billion annually. (Brown , Ashley, & Koh, 2018) (Tran, et al., 2022) A few of the pressure, chemical, and optical sensors that have been explored for non-invasive wound monitoring are currently on the market. However, a microsystem that can detect moisture, temperature, pH, and chemical biomarkers is required to have a more complete wound monitoring system. (Tran, et al., 2022) Continuous temperature increases of at least 1.11 degree C at the wound may be caused by infections or changes in metabolism, hence it is essential to frequently monitor the tissue temperature and moisture level to decide when to change the bandage or remove necrotic tissue. (Tran, et al., 2022) (Brown , Ashley, & Koh, 2018) Chronic wounds have a pH between 7.18 and 8.90, which results in a mildly basic environment that promotes bacterial colonisation. pH measurements alone are insufficient for infection monitoring, but when combined with additional biomarkers such uric acid (UA) and interleukin-6 (IL-6), they may be useful for detecting infections early on and the extent of the inflammatory phase. (Brown , Ashley, & Koh, 2018). (Pusta , Tertis, Cristea, & Mirel, 2022).The purpose of this project is to develop a flexible wearable system that includes inexpensive wireless temperature, moisture, and pH sensors as well as electrochemical biosensors for measuring, IL-6, and UA levels. This point-of-care (POC) system would act as a multifunctional in situ device that can diagnose wound parameters and regulate the infection at the wound site offering the potential to enhance available treatment options. The structure of the device would include an outer flexible textile substrate with integrated electronics, an (disposable) active layer on top with printed temperature, moisture, and electrochemical biosensors, and then a layer of absorbent material that would encounter the wound. Owing to its high electrical and thermal conductivity, high carrier mobility, functionality, and biocompatibility, liquid-phase exfoliated graphene will be addressed as the primary active material for temperature and electrochemical biosensors. (Davies, Tzalenchuk, Wiper, & Walton, 2016) For the temperature sensors the thermal conductivity of graphene will be exploited by printing graphene or graphene/temperature-sensitive polymers inks into sensor arrays (thermistors). (Ismail, Idris, & Abdullah, 2022) (Yan, Wang, & Lee, 2015) (Servati, Zou, Wang, Ko, & Servati, 2017). An optimal encapsulation method, biocompatible substrate material (i.e., polylactic acid (PLAA), cellulose, and chitosan) and correction method for signal susceptibility to motion and strain will be investigated. For the moisture sensor, electrodes made of conductive materials (i.e., graphene, Ag, and AgCl) will be taken into consideration. (McColl, MacDougal, Watret, and Connolly, 2009) When a small current is delivered to these electrodes by a portable metre, the electrical impedance across the sensor electrodes will allow the moisture level to be detected. As an alternative, ion-conductive polymeric electrodes (i.e., PEDOT: PSS, PPy, PANI) will be printed that, when in contact with the ion-rich exudate, will modify their ionic electronic conduction mechanism, extrapolating into impedance changes. (Tessarolo, et al., 2021) To detect chemical biomarkers and monitor pH levels, inkjet-printed EnFET (enzyme field-effect transistor devices) and ISFET (ion-selective field-effect transistor) will be investigated respectively.

慢性伤口是那些需要长达三个月的时间来治愈或在及时治疗后不显示改善的伤口，更有可能被细菌感染或殖民。当前，管理慢性伤口的最普遍的方法是视觉评估和诊断敷料，对医疗保健部门产生重大影响。慢性伤口影响美国570万人，每年耗资200亿美元。 （Brown，Ashley和Koh，2018年）（Tran等，2022）目前在市场上探索了用于非侵入性伤口监测的一些压力，化学和光学传感器。但是，需要检测水分，温度，pH和化学生物标志物的微型系统必须具有更完整的伤口监测系统。 （Tran等，2022）在伤口处的连续温度至少升高至少1.11度C可能是由感染或代谢变化引起的，因此，必须经常监测组织温度和水分水平以决定何时改变绷带或去除坏死组织。 （Tran等，2022）（Brown，Ashley和Koh，2018年）慢性伤口的pH值在7.18至8.90之间，这导致了一个温和的基本环境，从而促进细菌菌落。仅pH值测量不足对于感染监测，但是与其他生物标志物（例如尿酸（UA）和白介素6（IL-6））结合使用时，它们可能对早期和炎症阶段的久经感染可能很有用。 （Brown，Ashley和Koh，2018年）。 （Pusta，Tertis，Cristea和Mirel，2022年）。该项目的目的是开发一种柔性可穿戴系统，其中包括廉价的无线温度，水分和pH传感器以及用于测量，IL-6和UA的电化学生物传感器水平。这种护理点（POC）系统将充当多功能的原位装置，可以诊断伤口参数并调节伤口部位的感染，从而提供潜力，以增强可用的治疗选择。该设备的结构将包括带有集成电子设备的外部柔性纺织基板，顶部（一次性）有效层，带有印刷温度，水分和电化学生物传感器，然后是一层会遇到伤口的吸收性材料。由于其高电导率，高载流子迁移率，功能和生物相容性，液相去角质石墨烯将作为温度和电化学生物传感器的主要活性材料。 （Davies，Tzalenchuk，Wiper和Walton，2016年）对于温度传感器，将石墨烯的热导率通过将石墨烯或石墨烯/温度敏感的聚合物墨水打印到传感器阵列（热敏电阻）中来利用石墨烯的导热率。 （Ismail，Idris和Abdullah，2022年）（Yan，Wang和Lee，2015年）（Servati，Zou，Wang，Ko，＆Servati，2017年）。将研究一种最佳封装方法，生物相容性的底物材料（即聚乳酸（PLAA），纤维素和壳聚糖）和用于信号运动和应变的校正方法。对于水分传感器，将考虑由导电材料（即石墨烯，AG和AGCL）制成的电极。 （McColl，MacDougal，Watret和Connolly，2009年）当通过便携式仪表输送到这些电极时，传感器电极上的电阻抗将允许检测到水分水平。作为替代方案，将打印离子传导性聚合物电极（即PEDOT：PSS，PPY，PANI），当与富含离子的渗出液接触时，将修改其离子电子传导机制，将其外推成障碍物变化。 （Tessarolo等，2021年）将分别研究为检测化学生物标志物和监测pH值，并分别研究INZYME打印的ENFET（酶场效应晶体管设备）和ISFET（离子选择性晶体效应晶体管）。