Molecular principles for the biogenesis and assemble of carboxysomes

羧基体生物发生和组装的分子原理

• 批准号: 2888283
• 金额: --
• 依托单位: University of Liverpool
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2888283
• 关键词: Molecular; principles; biogenesis; assemble; carboxysomes

Organelles confine specific biochemical pathways within the cell to enhance metabolic efficiency, alleviate metabolic crosstalk, and facilitate spaciotemporal regulation of sequestered pathways. The carboxysome is a bacterial microcompartment that functions as a primitive protein-based organelle for CO2 fixation. It encapsulates the key CO2-fixing enzymes, Rubisco and carbonic anhydrase, within a semi-permeable protein shell. This elaborate architecture provides elevated CO2 levels around Rubisco for enhanced carbon fixation. The carboxysome is predominantly in oceanic cyanobacteria and many proteobacteria, and makes a great contribution to global primary productivity. Despite its importance on the global scale, our knowledge about how the a-carboxysome is generated and functionally maintained in bacterial cells is surprisingly limited. This PhD project is aimed at studying the molecular principles underlying the in situ biosynthesis, assembly, and regulation of carboxysomes in autotrophic microorganisms, using multidisciplinary techniques of molecular genetics, biochemistry, high-resolution microscopic imaging, structural biology, and synthetic biology. By characterising the architectural and functional properties of various carboxysomes in the native and synthetic forms, this project will elucidate in unprecedented detail how carboxysomes are formed and functionally modulated in cells to fulfil functions, and the diversity of carboxysomes from different organisms in specific niches. Advanced understanding of microcompartment self-assembly and functionality will inform bioengineering of metabolic organelles using synthetic biology and development of new nanomaterials and protein scaffolds for diverse biotechnological applications.

细胞器将细胞内的特定生化途径局限于增强代谢效率，减轻代谢串扰并促进隔离途径的时空调节。羧化体是一种细菌微型室，可作为基于原始蛋白质的细胞器的二氧化碳固定器。它封装了一个半渗透蛋白壳中的关键二氧化碳固定酶，rubisco和碳赤铁酶。这种精致的建筑为Rubisco周围提供了升高的二氧化碳水平，以增强碳固定。羧化体主要在海洋蓝细菌和许多蛋白质细菌中，对全球主要生产力做出了巨大贡献。尽管它在全球范围内的重要性，但我们对如何在细菌细胞中生成和功能维护的A-羧化体的了解非常有限。该博士学位项目旨在研究使用分子遗传学的多学科技术，生物化学，高分辨率微分辨率微观的，结构生物学和合成学和合成学和合成的多学科技术，研究自养微生物中羧化学的原位生物合成和调节的分子原理。通过表征本机和合成形式中各种羧化体的结构和功能特性，该项目将以前所未有的细节阐明如何形成羧化体并在细胞中调制功能以实现功能，以及从不同生物体中的盒子物种的多样性。对微型组门的自我组装和功能的深入了解将使用合成生物学以及用于多种生物技术应用的新纳米材料和蛋白质支架的新代谢细胞器的生物工程。