Gene expression in commensal tsetse symbionts

共生采采蝇共生体中的基因表达

• 批准号: 7244283
• 负责人: BRIAN L WEISS
• 金额: $ 5.2万
• 依托单位: YALE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-06-09 至 2008-06-08
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7244283
• 关键词: African Trypanosomiasis; Antibiotic Resistance; Antibiotics; Arthropod Vectors; BMAP-27; Bacteria; Bos taurus; Cattle; Characteristics; Chromosomal Insertion; Chromosomes; Chromosomes, Human, Pair 2; Disease; Electroporation; Evolution; Exhibits; Fellowship; Gene Expression; Generations; Genes; Genome; Genomics; Glossina (subgenus); Goals; Human; Immune response; Immune system; Individual; Infection; Life; Microinjections; Midgut; Monitor; Names; Peptides; Plasmids; Polymerase Chain Reaction; Procedures; Purpose; Recombinant Proteins; Recombinants; Refractory; Research; Resistance; Site; Southern Blotting; System; Transgenic Organisms; Trypanocidal Agents; Trypanosoma; Trypanosoma brucei brucei; Tsetse Flies; antimicrobial; attacin; base; design; fitness; fly; homologous recombination; improved; microorganism; neutrophil; novel; pathogen; protein expression; research study; vector

DESCRIPTION (provided by applicant): African sleeping sickness in humans is a potentially deadly disease caused by trypanosomes (Trypanosoma brucei spp.). Central to inhibiting the spread of this rapidly re-emerging disease is control of the arthropod vector, the tsetse fly (genus Glossina). Tsetse harbors a commensal endosymbiotic microorganism (Sodalis glossinidius) that lives in the midgut in close proximity to the trypanosomes. The goal of this research is to exploit this symbiotic flora for the purpose of decreasing tsetse's capacity to transmit trypanosomes. To accomplish this goal, I plan to improve upon an existing extra-chromosomal plasmid-based transformation procedure by using homologous recombination to incorporate 'effector molecule' genes into Sodalis' chromosome. 2 candidate molecules, with demonstrated trypanolytic activity, are available for this purpose: tsetse attacin and vertebrate BMAP-27. Recombinant Sodalis will be reintroduced to tsetse. These 'paratransgenic' flies and their offspring will then be monitored for changes in fitness, stability of recombinant protein expression and trypanosome vectorial capacity.

描述（由申请人提供）：人类中的非洲熟睡是由锥虫引起的潜在致命疾病（Brucei spp。）。抑制这种快速重新出现疾病的传播的核心是控制节肢动物载体Tsetse Fly（Glossina属）的控制。采摘者拥有共生的内共生微生物（Sodalis glossinidius），该生物与锥虫相近生活在中肠中。这项研究的目的是利用这种共生菌群，目的是降低采摘者传输锥虫的能力。为了实现这一目标，我计划通过使用同源重组将“效应分子”基因掺入索达利斯染色体中，以改善现有的基于染色体质粒的转化程序。 2个候选分子，具有锥溶活性，可用于此目的：采集Attacin和脊椎动物BMAP-27。重组索达利斯将被重新引入采用。然后将监测这些“副基因”苍蝇及其后代的适应性变化，重组蛋白表达的稳定性和锥形体矢量能力。