CORE--EXPERIMENTAL ANIMAL

核心--实验动物

基本信息

批准号：
7553672
负责人：
Jennifer Gordon
金额：
$ 25.58万
依托单位：
TEMPLE UNIV OF THE COMMONWEALTH
依托单位国家：
美国
项目类别：
财政年份：
资助国家：
美国
起止时间：
至
项目状态：
未结题

来源：
https://reporter.nih.gov/project-details/7553672
关键词：
Papovaviridae animal colony athymic mouse biomedical facility disease /disorder model gene targeting genetically modified animals genotype hamsters laboratory mouse magnetic resonance imaging medulloblastoma model design /development polymerase chain reaction virus related neoplasm /cancer

项目摘要

Core A: Experimental Animal Core In exploring this viral model for tumor pathogenesis in the brain, experiments involving animal models are an essential component in all three projects. Thoward this end, the goal of the Experimental Animal Core of this program is to support Projects 1 - 3. In this core, we will focus on the following five goals: (i) Generation and maintenance of JCV T-antigen transgenic mice which spontaneously develop cerebellar medulloblastomas. The mice will be utilized to provide tumor tissue for analysis and for therapeutic treatment as outlined in the individual projects. (ii) Generation and analysis of new lines of transgenic and knockout mice. Inducible JCV T-antigen transgenic will be generated, as well as mice with mutant p53 or beta-catenin. JCV T-antigen transgenic mice will be crossed with mice lacking tumor suppressors such as p53 and Rb family members. (iii) Inoculation of established cell lines along the flanks of Nude mice or syngeneic mice for in vivo evaluation of therapeutic strategies as outlined in Projects 1-3. (iv) Transplantation of established cell lines within the brain parenchyma of Nude or syngeneic mice for in vivo evaluation of therapeutic strategies as outlined in Projects 1-3. (v) Inoculation of either tumor tissue or cell lines along the flanks of Nude mice to evaluate tumorigenecity. Tumor tissue will be excised and passed to Core B for analysis and establishment of cell lines for distribution to Projects 1-3.

核心A：实验动物核心在探索大脑肿瘤发病机制的病毒模型时，涉及动物模型的实验是这三个项目的重要组成部分。为此，本项目实验动物核心的目标是支持项目1-3。在这个核心中，我们将重点关注以下五个目标： (i) 自发发展小脑髓母细胞瘤的 JCV T 抗原转基因小鼠的产生和维持。这些小鼠将用于提供肿瘤组织进行分析和治疗，如各个项目中所述。 (ii) 转基因和基因敲除小鼠新品系的产生和分析。将产生可诱导的 JCV T 抗原转基因，以及具有突变 p53 或 β-连环蛋白的小鼠。 JCV T 抗原转基因小鼠将与缺乏 p53 和 Rb 家族成员等肿瘤抑制因子的小鼠杂交。 (iii)沿着裸鼠或同系小鼠的侧腹接种已建立的细胞系，用于体内评估项目1-3中概述的治疗策略。 (iv) 将已建立的细胞系移植到裸鼠或同系小鼠的脑实质内，用于项目 1-3 中概述的治疗策略的体内评估。 (v)沿裸鼠胁腹接种肿瘤组织或细胞系以评估致瘤性。肿瘤组织将被切除并传递至 Core B 进行分析和建立细胞系，以分配给项目 1-3。