CORE--ANIMAL EXPERIMENTATION

核心——动物实验

• 批准号: 6588775
• 负责人: THOMAS S MCCORMICK
• 金额: $ 4.59万
• 依托单位: CASE WESTERN RESERVE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6588775
• 关键词: animal colony; athymic mouse; biomedical facility; carcinogenesis; genetic susceptibility; genetically modified animals; laboratory mouse; skin disorder; skin neoplasms

Rodents, in particular mice, are extensively used by research projects of several SDRC investigators. An animal core is thus an important component of the SDRC that has been providing expertise and training in the successful completion of animal experiments in a cost effective and highly predictable manner. There is considerable emphasis on skin cancer in the SDRC. There is evident by the fact that 1) five nationally funded projects of this faculty either conduct murine skin tumorigenicity experiments or utilize cutaneous tumors developed on murine skin for biochemical morphological , and molecular biological studies, 2) one of the pilot and feasibility proposed in this renewal application will conduct murine skin tumorigenesis experiments, 3) in the NIH funded training program in Investigative and Molecular Dermatology there are four training tracks, one of which emphasizes skin carcinogenesis. Out of the two current trainees supported by this training grant, one utilizes murine skin tumors and the other conducts animal experiments. The developing emphasis is to utilize chemically mutagenized mice which have clinical phenotypes for dermatitis, susceptibility to UV, cancer and blistering diseases. The additional areas of research in SDRC are a) structural biology and genetics of the skin, and b) immunodermatology. Several investigators conducting research in these areas do animal experimentation. This core has enabled SDRC investigators to conduct animal experimentation more efficiently. The core has four specific functions. The first function is to provide SDRC faculty with a) tissue, in particular tumors, from cutaneous tumor-bearing and age- and sex- matched normal mice, and b) homogenate, RNA, DNA, and single cell suspensions, from defined tumors of variously treated mice. The second function is to provide expertise in planning, procurement, and handling of specialized animals for experiments to be conducted by SDRC faculty. Within this objective, the core serves as a resource center in a) providing technical expertise and advise in producing transgenic and knockout mice, and b) conducting experiments on nude mice, e.g. tumor development and their histological verification. The third, which is a newly added function, relates to screening, maintaining and breeding chemically mutagenized mice with clinical phenotypes of skin disease of interest to SDRC investigators. These mice should prove valuable in dissecting complex multi-gene phenotypes. The fourth function of the core is to provide SDRC faculty with biostatistical expertise. For all, experiments involving animal work being conducted and planned for the near future, expertise in murine skin carcinogenesis, biochemistry, genetics, biostatisticians, histopathology and veterinary husbandry and care is needed. Together Core Director, Co-Directors and Consultants represent a team capable of providing expertise in all aspects of animal experimentation.

啮齿动物，特别是小鼠，被 SDRC 几位研究人员的研究项目广泛使用。因此，动物核心是 SDRC 的重要组成部分，它一直以具有成本效益和高度可预测的方式提供成功完成动物实验的专业知识和培训。 SDRC 非常重视皮肤癌。显而易见的是，1）该学院的五个国家资助项目要么进行小鼠皮肤致瘤性实验，要么利用小鼠皮肤上形成的皮肤肿瘤进行生化形态学和分子生物学研究，2）本研究中提出的试点和可行性之一更新应用程序将进行小鼠皮肤肿瘤发生实验，3）在 NIH 资助的研究和分子皮肤病学培训计划中有四个培训课程，其中之一强调皮肤致癌作用。在这项培训资助支持的两名目前的学员中，一名利用小鼠皮肤肿瘤，另一名进行动物实验。开发的重点是利用具有皮炎、紫外线易感性、癌症和水疱性疾病临床表型的化学诱变小鼠。 SDRC 的其他研究领域包括 a) 皮肤结构生物学和遗传学，以及 b) 免疫皮肤病学。在这些领域进行研究的几位研究人员进行了动物实验。这一核心使 SDRC 研究人员能够更有效地进行动物实验。核心有四个特定功能。第一个功能是为 SDRC 教员提供 a) 组织，特别是来自皮肤肿瘤和年龄和性别匹配的正常小鼠的肿瘤，以及 b) 来自确定的肿瘤的匀浆、RNA、DNA 和单细胞悬浮液。经过不同处理的小鼠。第二个职能是为 SDRC 教师进行的实验提供规划、采购和处理专用动物的专业知识。在这一目标中，核心作为一个资源中心，a）提供生产转基因和基因敲除小鼠的技术专业知识和建议，b）在裸鼠上进行实验，例如裸鼠。肿瘤的发展及其组织学验证。第三个是新增加的功能，涉及筛选、维持和培育具有 SDRC 研究人员感兴趣的皮肤病临床表型的化学诱变小鼠。这些小鼠在剖析复杂的多基因表型方面应该具有价值。该核心的第四个功能是为 SDRC 教师提供生物统计专业知识。 对于所有正在开展和计划在不久的将来进行的涉及动物工作的实验，需要鼠皮肤癌发生、生物化学、遗传学、生物统计学家、组织病理学以及兽医饲养和护理方面的专业知识。核心总监、联合总监和顾问共同组成了一个能够提供动物实验各个方面专业知识的团队。