Core--Genomics

核心--基因组学

• 批准号: 7217714
• 负责人: HOWARD J JACOB
• 金额: $ 50.95万
• 依托单位: MEDICAL COLLEGE OF WISCONSIN
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-07-01 至 2011-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7217714
• 关键词: angiotensin /renin /aldosterone hypertension; bioinformatics; biomedical facility; functional /structural genomics; genetic mapping; genetic polymorphism; genetic strain; genotype; inbreeding; molecular cloning; nucleic acid sequence

The Genomics Core, under the direction of Dr. Jacob, will serve Projects by Cowley, Roman, and Greene in the genotyping of the congenic animals, sequencing, and providing bioinformatics support for all the Projects and Cores. This Core generates all wet lab genomic data related to genotyping the rats to generate the congenic and subcongenic animals using simple sequence length polymorphisms (SSLPs), generation of SSLP markers, cloning, sequencing, single nucleotide polymorphism (SNP) detection and physical mapping (if needed). Dr. Jacob has extensive experience in all facets of this Core through his last 16 years of research. Dr. Jacob directs the Human and Molecular Genetics Center (HMGC) at the Medical College of Wisconsin (MCW). This center has in place all the personnel, equipment, robotics and laboratory information management systems required to meet the needs of this PPG. The genotyping component, highly automated, is set up to make early breeding decisions, which allows us to minimize the cost of rat housing. The Genomics Core will also direct many of the activities related to mutation detection and gene identification in Projects by Roman and Greene. Finally, gene hunting, high through-put sequencing and comparative genomics all require significant bioinformatics infrastructure. Dr. Jacob is the PI for the Rat Genome Database (RGD) and directs the Bioinformatics Program at MCW. As a result the analytical tools necessary for data analysis, gene identification and prediction, and gene expression analysis are all in place and operational.

基因组学核心在 Jacob 博士的指导下，将为 Cowley、Roman 和 Greene 的基因分型项目提供服务 对同类动物进行测序、测序，并为所有项目和核心提供生物信息学支持。这 Core 生成与大鼠基因分型相关的所有湿实验室基因组数据，以生成同类和 使用简单序列长度多态性（SSLP）的亚同类动物，SSLP标记的生成， 克隆、测序、单核苷酸多态性 (SNP) 检测和物理作图（如果需要）。博士。 Jacob 通过过去 16 年的研究，在该核心的各个方面都拥有丰富的经验。雅各布博士 指导威斯康星医学院 (MCW) 的人类和分子遗传学中心 (HMGC)。这 中心已配备所有人员、设备、机器人和实验室信息管理系统 以满足本 PPG 的需要。基因分型组件高度自动化，旨在尽早实现 繁殖决策，这使我们能够最大限度地减少老鼠饲养的成本。基因组学核心还将指导 Roman 和 Greene 项目中的许多活动与突变检测和基因鉴定相关。最后，基因 狩猎、高通量测序和比较基因组学都需要重要的生物信息学 基础设施。 Jacob 博士是大鼠基因组数据库 (RGD) 的 PI，并指导生物信息学 MCW 的计划。因此，数据分析、基因识别和分析所需的分析工具 预测和基因表达分析均已到位且可操作。