Biomarkers of Response to Environmental Stressors: Measurement of Environmental E

对环境压力源反应的生物标志物：环境 E 的测量

基本信息

批准号：
7363755
负责人：
Eva R Birnbaum
金额：
$ 30万
依托单位：
ICAGEN, INC.
依托单位国家：
美国
项目类别：
财政年份：
2007
资助国家：
美国
起止时间：
2007-09-15 至 2009-08-31
项目状态：
已结题

来源：
https://reporter.nih.gov/project-details/7363755
关键词：
Acute Address Amyotrophic Lateral Sclerosis Antibodies Arsenates Arsenic Arsenites Asthma Autistic Disorder Award Binding Biological Biological Assay Biological Markers Biological Products Blood Body Fluids Cadmium Chemicals Chronic Collaborations Collection Compatible Computer software Coomassie blue Copper Coupled Daily Data Development Devices Diabetes Mellitus Dietary Questionnaires Disease Disease susceptibility Dose Environment Environmental Exposure Epidemiologic Studies Exposure to Foundations Gel Genes Genetic Genome Genomics Genotype Goals Haplotypes Hour Human Human Genome Project Image Immunoassay Individual Invasive Investigation Iron Kinetics Knowledge Laboratories Lead Left Ligands Link Location Malignant Neoplasms Measurable Measurement Measures Mercury Metabolic Biotransformation Metabolic Pathway Metal exposure Metallothionein Metals Methods Micronutrients Monitor Participant Patients Pattern Pesticides Pharmaceutical Preparations Pharmacologic Substance Phase Population Population Study Predisposition Program Development Protein Array Protein Binding Proteins Proteome Proteomics Reading Relative (related person)Research Salts Sampling Selenium Simulate Spottings Staining method Stains Standards of Weights and Measures Surface System Technology Testing Thinking Title Toxic Environmental Substances Toxic effect Toxin Urine Xenobiotics Zinc adduct artist autism spectrum disorder base cohort commercialization concept dietary supplements drinking water environmental chemical environmental stressor gel electrophoresis gene environment interaction high throughput screening high throughput technology human disease lead contamination metal poisoning nanoparticulate nervous system disorder prototype research and development response scale up sensor stressor tool toxic metal toxicant

项目摘要

DESCRIPTION (provided by applicant) Caldera Pharmaceutical's will use their unique, R&D100-award-winning Reagentless Pharmacoproteomic Measurement (RPM) high-throughput technology to identify biomarkers of response to environmental stressors (BRES). Caldera developed RPM in collaboration with Los Alamos National Laboratory. RPM allows the identification and measurement of BRES consisting of protein-metal adducts and metabolites. We propose to develop BRES to define patterns of response to benefit the Gene Environment Initiative, focusing on xenobiotic biotransformation in human disease like Autism. Our BRES will lead to reliable measurements of exposures, which can be correlated with variable susceptibility to metal toxicity in the human population. This provides valuable information involving metabolic pathways for the Gene Environment Initiative (GEI). We will measure exposure to metal stressors in biospecimens by developing BRES that can identify exposure to particular chemical forms of toxic metals. Protein:metal adducts formed in individual patients may be correlated to genetic differences regulating metallothionein or other metallome proteins thought to be related to autism. RPM can monitor simultaneous exposure to toxins, such as arsenic, cadmium, lead, and mercury (thimerasol), as well as exposure to essential metals (zinc, iron, selenium, etc.). Individuals with autism may have varying genes that biotransform and detoxify metals differently, leading to varying accumulation and disease susceptibility.

描述（由申请人提供） Caldera Pharmaceutical将使用其独特的R＆D100奖授予试剂药物蛋白质组学测量（RPM）高通量技术来识别对环境压力源（BRES）反应的生物标志物。 Caldera与Los Alamos国家实验室合作开发了RPM。 RPM允许鉴定和测量由蛋白质金属加合物和代谢产物组成的BRE。我们建议开发BRE来定义反应模式以使基因环境倡议受益，重点是自闭症等人类疾病中的异生物生物转化。我们的培训将导致对暴露的可靠测量，这可能与人口中对金属毒性的可变敏感性相关。这提供了涉及基因环境计划（GEI）代谢途径的宝贵信息。我们将通过开发可以鉴定出可能暴露于特定化学形式的有毒金属的BRE来测量生物测量中的金属应激源。蛋白质：在个别患者中形成的金属加合物可能与调节金属硫蛋白或其他被认为与自闭症有关的金属硫蛋白或其他金属蛋白的遗传差异相关。 RPM可以监测毒素，例如砷，镉，铅和汞（Thimerasol），以及暴露于必需金属（锌，铁，硒等）。自闭症的个体可能具有不同的基因，这些基因对金属的生物转化和排毒的金属有所不同，从而导致累积和疾病敏感性变化。