MICROFLUIDIC EVAPORATION-BASED SAMPLE CONCENTRATOR

基于微流控蒸发的样品浓缩器

• 批准号: 6993833
• 负责人: Ronald Louis Bardell
• 金额: $ 10万
• 依托单位: MICROPLUMBERS MICROSCIENCES, LLC
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-09-30 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6993833
• 关键词: bacteria; bioassay; bioengineering /biomedical engineering; biomedical automation; biomedical equipment development; chemical property; computer program /software; computer simulation; consumable /disposable biomedical equipment; evaporation; fluorimetry; image processing; immunologic assay /test; microfluidics; miniature biomedical equipment; nanotechnology; nucleic acid quantitation /detection; protein quantitation /detection; rapid diagnosis; small molecule; video microscopy; virus

DESCRIPTION (provided by applicant): The detection of very dilute bioparticles using lab-on-a-chip technology presents a currently unsolved problem. The applicants will design and demonstrate a microfluidic concentrator device that uses an isothermal rapid evaporation process for the concentration of bacteria, viruses, and proteins. The biosample can be maintained at room temperature or at a somewhat elevated temperature throughout the concentration process, while the only constituents removed are the volatiles (i.e., fluids that have a high vapor pressure, primarily water). The process takes a sample volume of up to 20 milliliters and rapidly concentrates it to a final volume of 100 microliters. The disposable device will have dimensions of approximately 5 cm x 6 cm x 0.7 cm, which includes the sample reservoir. The concentrator is particularly powerful because it is generically applicable, does not depend on the characteristics of the dissolved or dispersed particles to be concentrated, and requires little or no external instrumentation. Further, the applicants will design and evaluate a chemical lysing structure based on exposure of a focused ribbon of bioparticles to diffusing lysing agents contained in a sheath stream. The lysing structure can be integrated with the evaporative concentrator as well as with other sample preparation subcircuits. All devices will be implemented as laser-microfabricated laminated microfluidic disposables. A successful Phase I will provide a generically applicable evaporative concentrator unit. After successful completion of Phase I, MicroPlumbers will apply for Phase II funding for the development of an integrated prototype comprising the evaporative concentrator, debris removal unit, lysing system, and an interface to a detection technology compatible with microfluidic devices.

描述（由申请人提供）：使用芯片实验室技术检测非常稀的生物颗粒是目前尚未解决的问题。申请人将设计并演示一种微流体浓缩装置，该装置使用等温快速蒸发过程来浓缩细菌、病毒和蛋白质。在整个浓缩过程中，生物样品可以保持在室温或稍微升高的温度，而唯一去除的成分是挥发物（即具有高蒸气压的液体，主要是水）。 该过程需要最多 20 毫升的样品体积，并快速将其浓缩至 100 微升的最终体积。一次性装置的尺寸约为 5 cm x 6 cm x 0.7 cm，其中包括样品储存器。该浓缩器特别强大，因为它具有普遍适用性，不依赖于待浓缩的溶解或分散颗粒的特性，并且需要很少或不需要外部仪器。 此外，申请人将基于生物颗粒的聚焦带暴露于鞘流中包含的扩散裂解剂来设计和评估化学裂解结构。裂解结构可以与蒸发浓缩器以及其他样品制备子电路集成。所有设备都将采用激光微加工层压微流体一次性用品。成功的第一阶段将提供普遍适用的蒸发浓缩器装置。 第一阶段成功完成后，MicroPlumbers 将申请第二阶段资金，用于开发集成原型，包括蒸发浓缩器、碎片清除装置、裂解系统以及与微流体设备兼容的检测技术的接口。