Dissecting heat stress responses in barley

剖析大麦的热应激反应

• 批准号: 2886133
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2886133
• 关键词: Dissecting; heat; stress; responses; barley

Background: Impact of changing climatic conditions on global crop production. We need to increase the population to feed the world but while reducing the reliance on fertiliser application. One solution is to develop varieties that have improved soil resource capture and thus improved yield under suboptimal conditions. Crop yields are globally affected by abiotic stresses such as drought, salinity and waterlogging. Ubiquitin-mediated proteolysis via the Plant Cysteine Oxidase (PCO) branch of the PRT6 N-Degron pathway controls the plant response by regulating the turnover of proteins involved in sensing and/or conferring tolerance to abiotic stresses. Root cortical senescence (RCS). Importance of cortical senescence towards this especially improving yield while reducing metabolic burden of maintaining cortical tissues under abiotic stresses. RCS is a very important breeding target for edaphic stress tolerance. The fundamental understanding about genes and mechanisms controlling this process is missing. Interestingly, we recently observed that mutants in PRT6 N-degron pathway have increased RCS. This project focuses on newly discovered and uncharacterized N-Degron pathway substrates to shed light on their specific downstream stress regulation functions, offering new opportunities for stress resilience research. We have previously identified two such substrates, BERF1 and RAF; this project will study their specific roles as key regulators of environmental stress. A recent paper showed RAF and BERF1 were upregulated in a transcriptional analysis, which indicated their important role of these gene in the development of root cortical senescence (RCS). Thus, in this DTP project we aim to dissect this to improve our fundamental understanding about genes controlling this process and identify novel genetic components/solutions that could help us develop varieties tolerant to abiotic stresses.Work plan: Year 1: Map the development of root cortical senescence during drought in the mutant and WT. Decide zones and timepoints for sampling roots and perform RNAseq of mutant vs WT under control and drought stress and sample different zones, including RCS formation to understand genes expressed. Prioritise some candidates' genes based on previous work and its relevance in drought, etc.Year 2-3: Develop CRISPR, RNAi, reporter lines to validate downstream targets of PRT6 N-degron pathway functioning in regulating root cortical senescence. Year 3-4. Perform drought experiment in soil columns in Hounsfield facility. Harvest roots for Laser ablation tomography and see whether RCS formation is impaired in the mutant and its impact on the cost benefit of forming RCS in the mutant vs WT. Also study shoot physiological parameters using PhenoSpex trait finder in the Hounsfield facility. Year 4: Deploy novel genetic solutions to breeding companies. Project output and impact: This project focuses on newly discovered and uncharacterized N-Degron pathway substrates to shed light on their specific downstream stress regulation functions, offering new opportunities for stress resilience research. This project therefore combines fundamental research into understanding the drivers for abiotic stress resilience, with application to generate resilient barley for deployment.

背景：改变气候条件对全球作物产量的影响。我们需要增加人口以养活世界，但在减少对肥料施用的依赖的同时。一种解决方案是开发有改善土壤资源捕获并因此在次优条件下提高产量的品种。农作物产量在全球范围内受到干旱，盐度和水池诸如的非生物压力的影响。泛素介导的蛋白水解通过PRT6 N-Degron途径的植物半胱氨酸氧化酶（PCO）分支通过调节涉及感应和/或赋予对依赖性耐心的耐受性的蛋白质的周转来控制植物反应。根皮质衰老（RCS）。皮质衰老对这种尤其提高产量的重要性，同时减轻了在非生物胁迫下维持皮质组织的代谢负担。 RCS是实现胁迫耐受性的非常重要的育种靶标。缺少对控制此过程的基因和机制的基本理解。有趣的是，我们最近观察到PRT6 N-Degron途径中的突变体增加了RC。该项目着重于新发现和未表征的N-Degron Pathway基板，以阐明其特定的下游压力调节功能，从而为压力弹性研究提供了新的机会。我们以前已经确定了两个这样的底物Berf1和Raf。该项目将研究他们作为环境压力的关键调节者的具体作用。最近的一篇论文显示，RAF和BERF1在转录分析中上调，这表明它们在根皮质衰老（RCS）发展中的重要作用。因此，在这个DTP项目中，我们旨在剖析这一点，以提高我们对控制这一过程的基因的基本理解，并确定可以帮助我们开发耐非生物压力的品种的新型遗传成分/解决方案。工作计划：1年：绘制突变体和WT中干旱期根本皮质衰老的发展。确定采样根和时间点的区域和时间点，并在控制和干旱应力下对突变体与WT进行RNASEQ，并采样不同的区域，包括RCS形成，以了解表达的基因。根据先前的工作及其在干旱中的相关性等优先考虑一些候选者的基因。 3-4年。在Hounsfield设施中的土壤柱中进行干旱实验。收获激光消融层析成像的根，看看突变体中的RCS形成是否受损，及其对在突变体与WT中形成RCS的成本益处的影响。还使用Hounsfield设施中的Phenpex特征查找器进行研究生理参数。 4年：将新型的遗传解决方案部署到育种公司。项目输出和影响：该项目着重于新发现和未表征的N-Degron Pathway基板，以阐明其特定的下游压力调节功能，为压力弹性研究提供了新的机会。因此，该项目结合了理解非生物压力弹性驱动因素的基本研究，并应用了用于部署的弹性大麦。