Barrier functions of the sugary cell coat: Understanding how extracellular signalling proteins and bacterial toxins navigate the cell surface

糖细胞外壳的屏障功能：了解细胞外信号蛋白和细菌毒素如何在细胞表面导航

基本信息

批准号：
2885385
负责人：
金额：
--
依托单位：
University of Leeds
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2023
资助国家：
英国
起止时间：
2023 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2885385
关键词：
Barrier functions sugary cell coat

项目摘要

Virtually every cell in the human body is surrounded by a carbohydrate-rich coat called the glycocalyx. The glycocalyx has been implicated in a variety of fundamental molecular and cellular processes including its ability to guide cell communication and behaviour by modulating the access of the extracellular signalling proteins, such as chemokines, to the cell surface. It also plays an important protective role as a barrier against pathogens, yet some bacterial toxins and viruses exploit the glycocalyx to gain entry to the host cells. Despite the omnipresence of the glycocalyx in the human body and its functional importance, its role has been elusive owing to the incredible complexity and hence overlooked as the tools for its investigation were missing.The biochemical and biophysical mechanisms of selective binding and transport of proteins and pathogens within the glycocalyx remain poorly understood. In this project, we are aiming to develop a mechanistic understanding of molecular diffusion within the glycocalyx by developing experimental glycocalyx models and new methods to probe the selective transport across the glycocalyx in molecular detail. The results of this project will provide new tools to further our fundamental understanding of glycocalyx functions that may in the future help tackling infectious diseases and control immune responses.

实际上，人体中的每个细胞都被称为糖蛋白的碳水化合物包围。糖蛋白已与各种基本分子和细胞过程有关，包括通过调节细胞外信号蛋白（例如趋化因子）进入细胞表面的访问来指导细胞通信和行为的能力。它也作为对病原体的障碍物起着重要的保护作用，但是一些细菌毒素和病毒利用糖蛋白以进入宿主细胞。尽管糖椰子在人体中的无所不知及其功能重要性，但由于缺少其研究的工具，其作用是难以捉摸的，因此被忽略了。蛋白质和蛋白质和病原体的选择性结合和运输的生物化学和生物物理机制仍然存在。在这个项目中，我们旨在通过开发实验性糖脂模型和新方法来对糖卵脂裂内的分子扩散进行机械理解，以分子细节探测跨糖囊的选择性转运。该项目的结果将提供新的工具，以进一步我们对糖脂功能的基本理解，这些功能将来可能有助于解决传染病并控制免疫反应。