Facilitating Access to Information on Human Proteins and

促进获取有关人类蛋白质和

• 批准号: 7291810
• 负责人: James Shaw
• 金额: --
• 依托单位: DIVISION OF BASIC SCIENCES - NCI
• 依托单位国家: 美国
• 资助国家: 美国
• 起止时间: 至
• 项目状态: 未结题
• 来源: https://reporter.nih.gov/project-details/7291810
• 关键词: Facilitating; Access; Information; Human; Proteins

The MPR web site http://mpr.nci.nih.gov contains information about human phosphorylation sites against which there are commercially available phospho-antibodies. The tool continues to be frequently used by scientists around the world. For example, it has been used from over 5000 ip addresses around the world. We have continued to add antibodies periodically.The user can gain access to the information in MPR in several ways: by browsing a list of all proteins for which phospho-antibodies are available; by searching for sites on an individual protein, or by browsing lists of phospho-antibodies from any particular company. Information provided for a site includes the exact sequence of surrounding residues (which is critical to unambiguous identification of the site of interest), a list of antibodies available against that site, several critical parameters for those antibodies, direct links to the supplier's web site information on each antibody; and links to pubmed citations related to that site. MPR also provides links to other web pages for each gene/protein that facilitates user exploration of resources including Genbank, Locuslink, CGAP, and Symatlas pertinent to their studies. Additional functionality provided is the ability to search for a site (and antibodies) based on the sequence of that site. Not only does that allow rapid/easy retrieval of the site information on that site, it also allows the user to survey sites of similar sequence that may be observed as cross-reactions or may have functional similarities. In addition, MPR now provides a curated collection of links that directs users to other particularly informative web cites relevant to protein phosphorylation.The tool we have developed for scanning proteins for PKC sites also continues to be used by many scientists. We have improved formatting and infrastructure that will facilitate future enhancements. The infrastructure we have developed for MPR has continued to facilitate our role in organizing information on phosphorylation sites for the CCR/BD collaboration. In addition, we have now constructed a prototype web-based version of KBTool, a knowledgebase program that we have been using internally for several years. We will begin testing it with a small group of external users.

MPR网站http://mpr.nci.nih.gov包含有关人类磷酸化位点的信息，其中有商业可用的磷酸化抗体。该工具继续被世界各地的科学家经常使用。例如，它已在全球5000多个IP地址中使用。我们继续定期添加抗体。用户可以通过多种方式访问​​MPR信息：通过浏览所有可用磷酸抗体的蛋白质列表；通过在单个蛋白质上搜索网站，或通过浏览任何特定公司的磷酸抗体清单。为站点提供的信息包括周围残基的确切顺序（这对于对感兴趣的位点的明确识别至关重要），该位点可用的抗体列表，这些抗体的几个关键参数，直接链接到供应商网站上每种抗体的网站信息；并链接到与该站点有关的PubMed引用。 MPR还为每个基因/蛋白质的其他网页提供了链接，这些链接促进了与他们的研究相关的GenBank，Locuslink，CGAP和Symatlas等资源的探索。提供的其他功能是能够根据该站点的序列搜索位点（和抗体）。这不仅允许在该站点上快速/易于检索网站信息，还允许用户调查相似序列的站点，这些序列可能被观察到交叉反应或可能具有功能相似之处。此外，MPR现在还提供了一个策划的链接集合，该链接将用户引导到其他特别有用的网络引用与蛋白质磷酸化相关的链接。我们开发的用于扫描PKC网站的蛋白质的工具也继续使用许多科学家使用。我们改进了格式和基础设施，可以促进未来的增强功能。我们为MPR开发的基础架构继续促进我们在组织CCR/BD协作的磷酸化站点的信息中的作用。此外，我们现在已经构建了基于Web的原型KBTool版本，KBTool是一个知识库程序，我们在内部使用了几年。我们将开始使用一小部分外部用户进行测试。