4000 Q TRAP LC/MS/MS shared mass spectrometer

4000 Q TRAP LC/MS/MS 共用质谱仪

• 批准号: 7034061
• 负责人: SARAH SPIEGEL
• 金额: $ 44.11万
• 依托单位: VIRGINIA COMMONWEALTH UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2007-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7034061
• 关键词: experience; fluid; human; ions; lipids; metabolism; teacher; university

DESCRIPTION (provided by applicant): Virginia Commonwealth University has a very active and growing critical mass of funded investigators working to understand how dynamic changes in bioactive lipids influence metabolism and the signaling pathways that regulate normal and pathological human cellular functions. Funds are requested for an Applied Biosystems 4000 Q TRAP(tm) LC/MS/MS System, a hybrid triple quadrupole/linear ion trap mass spectrometer coupled with a liquid chromatograph. This exceptionally flexible instrument combines the unique capabilities of the most sensitive triple quadrupole mass spectrometer available with the highest capacity ion trap to provide mass resolution, sensitivity, and dynamic response needed for structural characterization and quantitation of the diverse molecules that comprise the lipidome. As described in this application, 18 NIH-funded scientists and clinicians from 7 different departments involved in over 45 NIH- supported research programs have specific needs for this mass spectrometer. The 4000 Q TRAP will be operated as a shared research resource to provide essential support for our ongoing research programs and to facilitate new collaborative research initiatives for lipid identification, quantitation, and characterization, with particular emphasis on cell signaling and functional lipidomics. Providing a principal justification for this request, there is no mass spectrometer with the required capabilities on the Medical Center campus. The instrument will be administered as a VCU core resource under the direction of Professors Sarah Spiegel and William M. Grogan, who have extensive experience in lipid analysis and administration of core resources. Prof. Richard Moran will chair the Advisory Committee overseeing equitable use of the instrument for the benefit of funded investigators and the university research environment. The purchase of this mass spectrometer is essential to continuing development and realization of the full potential of lipidomics research toward better understanding of normal cellular function and human disease on the VCU Medical Center campus.

描述（由申请人提供）：弗吉尼亚州联邦大学拥有非常活跃和日益增长的临界质量，这些研究人员致力于了解生物活性脂质的动态变化如何影响代谢以及调节正常和病态人类细胞功能的信号传导途径。要求资金用于应用生物系统4000 Q陷阱（TM）LC/MS/MS系统，这是一种杂交三倍四倍/线性离子离子陷阱质谱仪与液相色谱仪的耦合。这种非常灵活的仪器结合了可用的最敏感三四极杆质谱仪的独特功能，并具有最高容量的离子陷阱，以提供质量分辨率，灵敏度和动态响应，以进行结构表征和定量构成脂质组的多元化分子。如本应用程序中所述，来自7个不同部门的NIH资助的科学家和临床医生与45多名NIH受支持的研究计划有关该质谱仪有特定的需求。 4000 Q TRAP将作为共享研究资源运行，以为我们正在进行的研究计划提供基本的支持，并促进新的合作研究计划，以进行脂质识别，定量和表征，并特别着重于细胞信号传导和功能性脂质词。为此请求提供主要理由，没有质谱仪在医疗中心校园内具有所需的功能。该仪器将在莎拉·斯皮格尔（Sarah Spiegel）教授和威廉·M·格罗根（William M. Grogan）的指导下作为VCU核心资源管理，他们在脂质分析和核心资源管理方面具有丰富的经验。理查德·莫兰（Richard Moran）教授将主持咨询委员会主席，以监督该工具的平等使用，以利用资助的研究人员和大学研究环境。购买该质谱仪对于持续发展和实现脂肪组学研究的全部潜力至关重要，以更好地理解VCU医疗中心校园的正常细胞功能和人类疾病。