Improving the therapeutic immunity of cancer vaccine with multi-adjuvant polymeric nanoparticles

多佐剂聚合物纳米粒子提高癌症疫苗的治疗免疫力

• 批准号: 2881726
• 金额: --
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2881726
• 关键词: Improving; therapeutic; immunity; cancer; vaccine

Head and neck squamous cell carcinoma (HNSCC) are an aggressive form of cancer attributes to 90% of all cancers in the head and neck area. Currently, chemo-immunotherapy is used as first line treatment for HNSCC patients. However, patients with advanced or recurrent HNSCC tumors respond poorly to this treatment, and there is therefore a need to develop a novel strategy to enhance the responsiveness of the HNSCC tumors to the current immunotherapy. Nanotechnology-based cancer vaccines offers an invaluable tool to tailor cancer vaccines to generate robust antitumor immune response against cancer cells in comparison to conventional delivery techniques. Polymer based nanoparticles are widely considered as better a nanoparticle system since they are non-toxic and have less side effects. The aim of this project is to develop a polymer-based nanoparticles for the delivery of adjuvants (immune activators) and antigens and assess the antitumor-immune activity in the HNSCC mouse model. Under the guidance of Prof. Sebastien Perrier, the student (year 1) will design the polymeric nanoparticles and characterize their properties in vitro. Then, under the supervision of Dr. Shashi Prasad Rama, lead material candidates will be assessed in vivo (year 2 and 3), by developing HNSCC mouse models and characterizing the antitumor immunity. The students will learn about cutting edge tools to develop and characterize polymeric nanoparticles and further will gain skills on development of in vivo tumor animal models and various molecular biology techniques such as flow cytometry, ELISA, and immunohistochemistry etc. This project will be highly multidisciplinary, being based both at the University of Warwick and the University Hospital and provide an excellent support for the training of a student from materials science to preclinical research.

头颈部鳞状细胞癌（HNSCC）是一种侵略性形式的癌症属性，占头颈部所有癌症的90％。目前，将化学免疫疗法用作HNSCC患者的第一线治疗。但是，患有晚期或经常性HNSCC肿瘤的患者对这种治疗的反应较差，因此需要制定一种新型策略来增强HNSCC肿瘤对当前免疫疗法的反应性。基于纳米技术的癌症疫苗提供了一种宝贵的工具来调整癌症疫苗，以与常规递送技术相比，针对癌细胞产生强大的抗肿瘤免疫反应。基于聚合物的纳米颗粒被广泛认为是更好的纳米颗粒系统，因为它们是无毒的并且具有较少的副作用。该项目的目的是开发基于聚合物的纳米颗粒，用于递送佐剂（免疫激活剂）和抗原，并评估HNSCC小鼠模型中的抗肿瘤 - 免疫活性。在塞巴斯蒂安·佩里尔（Sebastien Perrier）教授的指导下，学生（第1年）将设计聚合物纳米颗粒并在体外表征其特性。然后，在Shashi Prasad Rama博士的监督下，将通过开发HNSCC小鼠模型并表征抗肿瘤免疫力，在体内评估铅材料候选物。学生将学习开发和表征聚合物纳米颗粒的尖端工具，并进一步获得在体内肿瘤动物模型发展和各种分子生物学技术（例如流式细胞仪，ELISA和免疫组织化学等）等各种分子生物学技术等方面的技能。该项目将是高度多学科的，是沃威克大学的一项培训，并提供了一项培训的培训。