Elucidating the role of a novel virus-induced nuclear condensate in non-coding RNA biogenesis and the importance of the royal family of m6A reader pro

阐明新型病毒诱导的核凝聚物在非编码RNA生物发生中的作用以及m6A阅读器亲王室的重要性

• 批准号: 2878039
• 金额: --
• 依托单位: University of Leeds
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2878039
• 关键词: Elucidating; role; novel; virus; induced

The ability of non-coding RNAs (ncRNAs) to regulate gene expression offers unique possibilities for viruses to modulate virus gene expression. We have recently shown that the herpesvirus, KSHV, can manipulate the levels of host cell circular RNAs to enhance its own replication, by regulating the activity of other ncRNA species in so-called ncRNA regulatory networks. However, how KSHV regulates circRNA biogenesis to dysregulate their levels during infection is unknown. In this project I will examine (i) the role of virus-induced nuclear condensates in circRNA biogenesis and (ii) the role of host cell m6A machinery, particularly novel m6A reader proteins, utilised by viruses to regulate both viral and cellular ncRNA and mRNA processing during infection. Therefore, the project aims to better understanding the roles of ncRNA and m6A in virus replication and how gene expression is regulated post-transcriptionally during infection.

非编码RNA（NCRNA）调节基因表达的能力为调节病毒基因表达的病毒提供了独特的可能性。我们最近表明，疱疹病毒KSHV可以通过调节所谓的NCRNA调节网络中其他NCRNA物种的活性来操纵宿主细胞循环RNA的水平，从而增强其自身的复制。然而，KSHV如何调节circrna生物发生在感染过程中的水平失调是未知的。在这个项目中，我将研究（i）病毒诱导的核冷凝物在ciRCRNA生物发生中的作用，以及（ii）宿主细胞M6A机械的作用，尤其是新型的M6A读取器蛋白，该蛋白使用病毒来调节病毒和细胞NCRNA和mRNA感染期间的处理。因此，该项目旨在更好地理解NCRNA和M6A在病毒复制中的作用，以及在感染期间转录后如何调节基因表达。