ULTRAFAST TIME-RESOLVED CRYSTALLOGRAPHY OF SCAPHARCA DIMERIC HEMOGLOBIN

甲鱼二聚体血红蛋白的超快时间分辨晶体学

• 批准号: 7181914
• 负责人: WILLIAM E ROYER
• 金额: $ 3.83万
• 依托单位: UNIVERSITY OF CHICAGO
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-08-01 至 2006-07-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7181914
• 关键词: biomedical resource; structural biology

DESCRIPTION (provided by applicant): We will continue to operate BioCARS, a nationally-available research resource for synchrotron-based macromolecular crystallography at the Advanced Photon Source, Argonne National Laboratory. The facility contains three experimental stations, one served by either an undulator or a wiggler insertion device source and two served by a bending magnet source, all of which can be used simultaneously. All stations and the associated control areas are embedded in a BL3-level biosafety facility, to permit the safe study of, for example, pathogenic human viruses. Research by users has successfully and productively concentrated on de novo structure determination by MAD phasing; on the collection of accurate, high resolution, monochromatic data; on the study of crystals with large unit cell dimensions; and on time-resolved crystallography on time scales down to a few nanoseconds. We will continue to emphasize these areas and to support the most challenging user experiments, through our experienced technical and scientific staff. In a facility that is unique nationally and one of three internationally, we will further develop time-resolved crystallography in both core and collaborative research, largely on the insertion device station. Advances in this scientific technique have led to much broader user interest in applying it to a wide variety of systems, since it offers new insights into biological mechanism and function. Upgrades will be made to the X-ray beam lines, both to replace those older components damaged by radiation and by heating and to afford smaller focal spots that are better matched to the smaller crystals that users wish to examine. Detector and data acquisition and control software will be improved, both to support collaborative projects in structural genomics that demand high data throughput, and to enable telecrystallography, in which the user can participate effectively in all aspects of the synchrotron experiment but need not be physically present at BioCARS. Strategies for improving the diffraction quality of crystals at both room and cryogenic temperatures by annealing will be implemented. In all cases, these developments are aimed at enhancing the throughput of excellent, high-resolution diffraction data and solved scientific problems, by satisfied users.

描述（由申请人提供）：我们将继续操作BioCars，这是Argonne National Laboratory的高级光子来源的基于同步加速器的大分子晶体学的全国可用研究资源。该设施包含三个实验站，一个由波动器或Wiggler插入装置源提供，两个由弯曲磁铁源提供，所有这些都可以同时使用。所有站点和相关的控制区域都嵌入BL3级生物安全设施中，以允许对病原体病毒进行安全研究。 用户的研究成功，有效地专注于通过疯狂的逐步确定从头结构的确定。关于准确，高分辨率，单色数据的收集；在研究具有较大晶胞尺寸的晶体时；并在时间分辨的晶体学上按时间扩展到几纳秒。我们将继续强调这些领域，并通过我们经验丰富的技术和科学人员来支持最具挑战性的用户实验。在国内独特的设施中，我们将在核心和协作研究中进一步开发时间分辨的晶体学，主要是在插入设备站。这种科学技术的进步使用户对将其应用于各种系统的兴趣更加广泛，因为它为生物学机制和功能提供了新的见解。 将对X射线光束线进行升级，以取代那些因辐射而损坏的旧组件，并通过加热和提供较小的焦点斑点，这些焦点与用户希望检查的较小晶体更匹配。探测器和数据采集和控制软件将得到改善，既可以支持需要高数据吞吐量的结构基因组学中的协作项目，又可以启用远程结构术，其中用户可以有效地参与同步体实验的各个方面，但不需要在bioCars上进行物理存在。通过退火来改善房间和低温温度的晶体衍射质量的策略将实施。在所有情况下，这些发展旨在增强满意的用户的出色高分辨率衍射数据和解决科学问题的吞吐量。