Engineering synthetic post-transcriptional regulatory circuits in S. cerevisae for co-ordinated gene expression

在酿酒酵母中设计合成转录后调节电路以协调基因表达

• 批准号: 2877491
• 金额: --
• 依托单位: University of Warwick
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2019
• 资助国家: 英国
• 起止时间: 2019 至 无数据
• 项目状态: 已结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2877491
• 关键词: Engineering; synthetic; post; transcriptional; regulatory

This project will explore options to develop novel forms of posttranscriptional circuitry for use in synthetic biology. In order to do this, an array of components, including RNA-binding proteins, RNA binding target motifs, degrons, effectors of these components, and other entities, will be studied in different combinations and tested in a range of novel scenarios. The various components will be characterised in detail and then their behaviour will be studied in the context of newly-constructed circuits. One of the contexts explored will be that of regulated expression of heterologous genes, for example genes involved in the biosynthesis of mycosporin-like amino acids (MAAs).In addition, the project will examine the benefits of combining transcriptional and posttranscriptional control elements in synthetic circuitry.

该项目将探索开发用于合成生物学的新型转录后电路的选择。为了做到这一点，一系列组件，包括RNA结合蛋白、RNA结合靶基序、降解决定子、这些组件的效应子和其他实体，将以不同的组合进行研究，并在一系列新的场景中进行测试。将详细描述各种组件的特性，然后在新建电路的背景下研究它们的行为。探索的背景之一是异源基因的调节表达，例如参与类菌孢菌素氨基酸（MAA）生物合成的基因。此外，该项目将研究在合成中结合转录和转录后控制元件的好处。电路。