Exploration of streptococcal carbohydrates for glycoconjugate vaccine development

链球菌碳水化合物用于糖复合物疫苗开发的探索

基本信息

批准号：
2876749
负责人：
金额：
--
依托单位：
University of Dundee
依托单位国家：
英国
项目类别：
Studentship
财政年份：
2023
资助国家：
英国
起止时间：
2023 至无数据
项目状态：
未结题

来源：
https://gtr.ukri.org/projects?ref=studentship-2876749
关键词：
Exploration streptococcal carbohydrates glycoconjugate vaccine

项目摘要

Strep A bacteria are human exclusive pathogens that are responsible for more than 700 m cases of infection globally per annum, with > 500,000 patients dying. Antimicrobial resistance increases in invasive Strep A isolates. Novel therapeutic options must be considered and the Dorfmueller laboratory has functionally build a glycoconjugate vaccine production platform that provides novel opportunities. The student will explore an E. coli production platform to test the feasibility to build several streptococcal proteins decorated with a species-specific carbohydrate. The student will be engaged in molecular biology and biochemistry, protein purification and analysis. In addition to Strep A bacteria, many other human and veterinary streptococcal bacteria produce related carbohydrates that are yet to be explored as vaccine candidates. The student will have the task to investigate the feasibility to engineer and build these carbohydrates in E. coli and characterise them for their composition and ability to be immunogenic. The student will also have the opportunity to build novel 'vehicles' for this new generation of carbohydrate vaccine candidates. In addition to expert training and advice in molecular biology, synthetic and glycan biology, the student will have the opportunity to communicate their project via different channels, including seminar presentations and public engagements activities.

链球菌A细菌是人类专属的病原体，每年全球感染的700 m以上，> 500,000例死亡。侵入性链球菌A分离株的抗菌耐药性增加。必须考虑新颖的治疗选择，Dorfmueller实验室在功能上建立了一个糖缀合物生产平台，可提供新的机会。该学生将探索一个大肠杆菌生产平台，以测试建造几种用物种特异性碳水化合物装饰的链球菌蛋白的可行性。该学生将从事分子生物学和生物化学，蛋白质纯化和分析。除了链球菌A细菌外，许多其他人类和兽医链球菌细菌还会产生相关的碳水化合物，这些碳水化合物尚待探索为候选疫苗。该学生将承担调查设计和在大肠杆菌中建造这些碳水化合物的可行性的任务，并以它们的成分和免疫原性的能力为特征。该学生还将有机会为新一代的碳水化合物疫苗候选者建造新颖的“车辆”。除了分子生物学，合成和聚糖生物学方面的专家培训和建议外，该学生还将有机会通过不同的渠道（包括研讨会演讲和公共参与活动）来传达其项目。