CRYSTALLOGRAPHIC STUDIES OF FRAGMENTS FROM REGULATED MYOSIN

调节肌球蛋白片段的晶体学研究

• 批准号: 7181013
• 负责人: CAROLYN COHEN
• 金额: $ 2.61万
• 依托单位: CORNELL UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2006-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7181013
• 关键词: biomedical resource; structural biology

DESCRIPTION (provided by applicant): MacCHESS, a synchrotron radiation Research Resource for macromolecular crystallography, has the overall goal of advancing the frontiers of structural biology through innovative technical research and development and user support. MacCHESS leverages the National Science Foundation investments in the Cornell High Energy Synchrotron Source (CHESS), which maintains the synchrotron radiation laboratory, and the Laboratory for Elementary Particle Physics (LEPP), which operates the storage ring. MacCHESS technical developments are driven by both Core and Collaborative research projects involving a broad range of macromolecules. In addition to performing technical R&D and Core research, MacCHESS provides specialized instrumentation for macromolecular crystallography and a staff for user training and support. MacCHESS has established itself as one of the most productive facilities in the world for macromolecular crystallography, with almost 437 papers published during the past five years as a result of MacCHESS related technical research and development, Core and Collaborative research and Service. More than 72 of these papers were published in the high visibility journals, Science, the Nature journals, and Cell. Three CHESS stations, A-1, F-1 and F-2, are used for macromolecular crystallography experiments. During the next five years, MacCHESS technical R&D will focus on (1) microcrystallography, (2) membrane protein crystallography, (3) large unit cell structures, (4) ultrahigh resolution diffraction, (5) high throughput crystallography, (6) crystallographic software and phasing techniques and (7) crystallographic beamline hardware development. Each of these technical R&D efforts is driven by the challenging problems presented by collaborating investigators who study the atomic structure of molecules and complexes of great current interest to medicine and biology, including membrane proteins, regulatory protein complexes, nucleic acid/protein complexes, protein drug interactions and viruses. Technical developments resulting from MacCHESS research are freely available to the scientific community and disseminated through meetings, workshops, publications, web distributions and collaborations with other synchrotron sources.

描述（由申请人提供）：MacChess是一种用于大分子晶体学的同步加速器辐射研究资源，其总体目标是通过创新的技术研究和开发和用户支持来推进结构生物学的前沿。 MacChess利用了维护同步加速器辐射实验室的康奈尔高能同步器（Chess）的国家科学基金会的投资，以及操作存储环的基本粒子物理（LEPP）实验室。 MacChess的技术发展是由涉及各种大分子的核心和协作研究项目驱动的。除了进行技术研发和核心研究外，MacChess还为大分子晶体学和用户培训和支持的人员提供专门的仪器。 MacChess已将自己确立为世界上最有生产力的大分子晶体学设施之一，由于MacChess相关的技术研究与开发，核心和协作研究和服务，在过去五年中发表了近437篇论文。这些论文中有72篇发表在高知名度期刊，科学，自然期刊和细胞中。三个国际象棋站A-1，F-1和F-2用于大分子晶体学实验。在接下来的五年中，MacChess技术研发将集中在（1）微晶学，（2）膜蛋白晶体学，（3）大型单元细胞结构，（4）超高分辨率衍射，（5）高吞吐量晶体学，（5）高吞吐量晶体学，（6）晶体学软件和浮标技术和（7）晶体图形束线硬件开发。这些技术研发工作中的每一个都是由研究人员提出的具有挑战性的问题驱动的，这些研究人员研究了分子的原子结构和对医学和生物学的巨大兴趣的原子结构，包括膜蛋白，调节蛋白质复合物，核酸/蛋白质复合物，蛋白质药物相互作用和病毒。由MacChess研究产生的技术发展可供科学界免费获得，并通过会议，研讨会，出版物，网络分布以及与其他同步源来源的合作进行传播。