COBRE: RW HOSP: P2 MYOGENIC POTENTIAL OF BONE MARROW CELLS

COBRE：RW HOSP：骨髓细胞的 P2 生肌潜能

• 批准号: 7171262
• 负责人: MEHRDAD ABEDI
• 金额: $ 19.81万
• 依托单位: ROGER WILLIAMS HOSPITAL
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2006-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7171262
• 关键词: bone marrow; cell differentiation; hematopoietic stem cells

DESCRIPTION (provided by applicant): This COBRE grant has 4 major objectives: 1.) The development of a strong mentoring group of established investigators; 2.) The enhancement of infrastructure support through the development of core laboratories, administrative support, and resources; 3.) The recruitment and retention of young and established faculty at Roger Williams Medical Center (RWMC) and collaborating Providence institutions (Brown, Miriam Hospital, and Rhode Island Hospital) in order to continue the establishment of a major stem cell research center for the state, region, and country; and 4.) To define the capacity of marrow cells to produce skin and muscle cells, and to define the clinical potential of these approaches in murine wound healing and muscular dystrophy models, and to establish Vector Systems to use siRNA to inhibit stem cell transcriptional pathways and to assess the effect on the stem cell phenotype, beginning with PU.1. We have assembled an experienced group of scientists who will mentor 3 promising young investigators and are prepared to mentor others in different research areas. The work is thematically coordinated around stem cell biology and plasticity and transcriptional regulation of stem cells and the potential for using siRNA to redirect stem cell differentiation. The 3 projects and their P.I.s are: Project 1 - Dr. Evangelos Badiavas - studies on the capacity of marrow cells to transdifferentiate (or convert) to skin cells and heal wounds. Project 2 - Dr. Mehrdad Abedi - the capacity of marrow cells to produce skeletal muscle cells and treat muscle disorders. Project 3 - Dr. Bharat Ramratnam - Stem cell gene modulation by RNAi. These scientific projects are supported by an Administrative Core, A Cell Sorter/Flow Cytometry Clore, and a Cell Phenotyping Core. Plans are outlined for continued mentoring of junior P.l.s, and specific approaches for evaluating the progress of the P.I.s. and a plan to specifically guide the P.l.s to RO1 funding is included. Institutional commitment is strong and not dependent on COBRE funding. Plans are also outlined for the recruitment and development of new junior faculty to the institutions and COBRE. The award of this COBRE application would effectively facilitate the continued development of The Center for Stem Cell Biology. This grant also has real promise in expanding our understanding of stem cell plasticity and stem cell transcriptional regulation and developing pre-clinical models for wound healing and muscular dystrophy.

描述（由申请人提供）： 这项 COBRE 资助有 4 个主要目标： 1.) 建立一个由知名研究人员组成的强大指导小组； 2.) 通过发展核心实验室、行政支持和资源来加强基础设施支持； 3.) 罗杰威廉姆斯医学中心 (RWMC) 和普罗维登斯合作机构（布朗医院、米里亚姆医院和罗德岛医院）招募和保留年轻和成熟的教职人员，以便继续为人类建立一个主要的干细胞研究中心州、地区和国家； 4.) 确定骨髓细胞产生皮肤和肌肉细胞的能力，确定这些方法在小鼠伤口愈合和肌营养不良模型中的临床潜力，并建立载体系统以使用 siRNA 抑制干细胞转录途径并从 PU.1 开始评估对干细胞表型的影响。 我们组建了一支经验丰富的科学家团队，他们将指导 3 名有前途的年轻研究人员，并准备指导不同研究领域的其他人。 这项工作的主题围绕干细胞生物学、干细胞的可塑性和转录调控以及使用 siRNA 重定向干细胞分化的潜力进行协调。 这 3 个项目及其主要负责人是： 项目 1 - Evangelos Badiavas 博士 - 研究骨髓细胞转分化（或转化）为皮肤细胞和治愈伤口的能力。 项目 2 - Mehrdad Abedi 博士 - 骨髓细胞产生骨骼肌细胞和治疗肌肉疾病的能力。 项目 3 - Bharat Ramratnam 博士 - 通过 RNAi 调节干细胞基因。 这些科学项目由管理核心、细胞分选仪/流式细胞仪 Clore 和细胞表型分析核心支持。 概述了继续指导初级 P.I.s 的计划，以及评估 P.I.s. 进度的具体方法。其中还包括一项专门指导 P.l.s 获得 RO1 资金的计划。 机构承诺是强有力的，并且不依赖于 COBRE 资金。 还概述了为各机构和 COBRE 招募和培养新初级教师的计划。 此次COBRE申请的获奖将有效促进干细胞生物学中心的持续发展。 这笔资助还有望扩大我们对干细胞可塑性和干细胞转录调控的理解，并开发伤口愈合和肌营养不良症的临床前模型。