CORE--AGING TRANSGENIC RODENT/PATHOLOGY

核心——转基因啮齿动物的衰老/病理学

• 批准号: 6948014
• 负责人: Evan T Keller
• 金额: $ 5.8万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6948014
• 关键词: aging; animal colony; animal old age; biomedical facility; genetically modified animals; genotype; laboratory mouse; mutant; pathology

The Aging Transgenic Rodent/Pathology Core (ATRPC) is directed by Evan Keller. The major purpose of the ATRPC is to provide Nathan Shock Center (NSC) and University of Michigan (UM) scientists with aged mice of the genotypes that provide otherwise unavailable opportunities to investigate the basic biology of aging or the pathophysiology of age-related disease states. A facility such as the ATRPC operated by the NSC at the UM does not exist anywhere else in the country. The ATRPC' s MTRC is primarily dedicated to exploiting the wide range of mutant and transgenics currently available, but not yet studied in the context of aging, for gerontological research . The ATRPC functions as a service core with the following specific aims: 1) the support of the per diem costs of aging a wide variety of mutant/transgenic rodents, (2) facilitating gross necropsy and histopathology of age-related lesions for genetically ~ modified mice, and (3) creating a database of the genetically -modified mice that are available for NSC and UM investigators. Over the previous five years of support, the ATRPC as supported thirteen projects with 12 faculty sponsors. Most projects were funded for 2 years, which was the usual time required to obtain mice at the ages required for the study. The projects funded during the current granting period included: evaluating aging effects on methylation in a methyltransferase knockout mice, determining aging effects on a humanized androgen receptor in mice, evaluation of wnt with age in bone and evaluation of estrogen receptor mutations with age. Several of these projects have led to publications and grant applications. The ATRPC has had a significant impact on the use of genetically-modified mice for NSC scientists on the UM campus and among their colleagues at UM and elsewhere.

衰老的转基因啮齿动物/病理核心（ATRPC）由埃文·凯勒（Evan Keller）指导。 ATRPC的主要目的是提供内森休克中心（NSC）和密歇根大学（UM）科学家，具有基因型的老年小鼠，这些小鼠提供了一些无法获得的机会，可以研究衰老的基本生物学或与年龄相关疾病状态的病理生理学。 NSC在UM运营的ATRPC等设施在该国其他任何地方都不存在。 ATRPC的MTRC主要致力于利用当前可用的广泛突变体和转基因，但尚未在衰老的背景下进行研究。 ATRPC充当以下特定目的的服务核心： 1）支持多种突变/转基因啮齿动物的衰老成本，（2）促进与年龄相关的病变的严重性死灵病和组织病理学的基因〜修饰的小鼠，以及（3）创建适用于NSC和UM研究员的遗传改良小鼠的数据库。在过去五年的支持中，ATRPC作为13个项目提供了支持，并提供了12位教职赞助商。大多数项目都资助了2年，这是在研究所需的年龄中获得小鼠所需的通常时间。在当前授予期间资助的项目包括：评估甲基转移酶敲除小鼠甲基化的衰老影响，确定对小鼠人性化雄激素受体的衰老影响，骨骼年龄和骨骼年龄评估雌激素受体突变随着年龄的年龄的评估。其中一些项目导致出版物和授予申请。 ATRPC具有重要的 对UM校园内的NSC科学家以及UM和其他地方的同事中的NSC科学家使用遗传改性小鼠的影响。