CORE--AGING TRANSGENIC RODENT/PATHOLOGY

核心——转基因啮齿动物的衰老/病理学

• 批准号: 6948014
• 负责人: Evan T Keller
• 金额: $ 5.8万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6948014
• 关键词: aging; animal colony; animal old age; biomedical facility; genetically modified animals; genotype; laboratory mouse; mutant; pathology

The Aging Transgenic Rodent/Pathology Core (ATRPC) is directed by Evan Keller. The major purpose of the ATRPC is to provide Nathan Shock Center (NSC) and University of Michigan (UM) scientists with aged mice of the genotypes that provide otherwise unavailable opportunities to investigate the basic biology of aging or the pathophysiology of age-related disease states. A facility such as the ATRPC operated by the NSC at the UM does not exist anywhere else in the country. The ATRPC' s MTRC is primarily dedicated to exploiting the wide range of mutant and transgenics currently available, but not yet studied in the context of aging, for gerontological research . The ATRPC functions as a service core with the following specific aims: 1) the support of the per diem costs of aging a wide variety of mutant/transgenic rodents, (2) facilitating gross necropsy and histopathology of age-related lesions for genetically ~ modified mice, and (3) creating a database of the genetically -modified mice that are available for NSC and UM investigators. Over the previous five years of support, the ATRPC as supported thirteen projects with 12 faculty sponsors. Most projects were funded for 2 years, which was the usual time required to obtain mice at the ages required for the study. The projects funded during the current granting period included: evaluating aging effects on methylation in a methyltransferase knockout mice, determining aging effects on a humanized androgen receptor in mice, evaluation of wnt with age in bone and evaluation of estrogen receptor mutations with age. Several of these projects have led to publications and grant applications. The ATRPC has had a significant impact on the use of genetically-modified mice for NSC scientists on the UM campus and among their colleagues at UM and elsewhere.

衰老转基因啮齿动物/病理学核心 (ATRPC) 由 Evan Keller 领导。 ATRPC 的主要目的是为内森休克中心 (NSC) 和密歇根大学 (UM) 的科学家提供具有基因型的老年小鼠，这些基因型为研究衰老的基本生物学或与年龄相关的疾病状态的病理生理学提供了其他机会无法获得的机会。国家安全委员会在密西根大学运营的 ATRPC 等设施在美国其他任何地方都不存在。 ATRPC 的 MTRC 主要致力于利用目前可用的各种突变体和转基因技术，但尚未在衰老背景下进行研究，以进行老年学研究。 ATRPC 作为服务核心，具有以下具体目标： 1）支持各种突变/转基因啮齿动物衰老的每日费用，（2）促进基因改造小鼠与年龄相关病变的大体尸检和组织病理学，以及（3）创建基因数据库 -可供 NSC 和 UM 研究人员使用的改良小鼠。在过去五年的支持中，ATRPC 支持了 13 个项目，有 12 名教师赞助。大多数项目的资助期限为两年，这是获得研究所需年龄的小鼠通常所需的时间。当前资助期间资助的项目包括：评估衰老对甲基转移酶敲除小鼠甲基化的影响、确定衰老对小鼠人源化雄激素受体的影响、评估骨中wnt随年龄的变化以及评估雌激素受体突变随年龄的变化。其中一些项目已发表并获得资助申请。 ATRPC 取得了重大成果 对密歇根大学校园内的国家安全委员会科学家以及他们在密歇根大学和其他地方的同事使用转基因小鼠的影响。