CORE--CONTRACTILITY

核心——收缩性

• 批准号: 6948017
• 负责人: JOHN Arthur FAULKNER
• 金额: $ 7.3万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-07-01 至 2010-06-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6948017
• 关键词: aging; biomedical facility; muscle contraction; striated muscles

Skeletal muscle contractility is defined as the capability of the contractile proteins in a muscle to interact and generate force. All body movements are dependent on contractions of skeletal muscles. Consequently, any decline in skeletal muscle contractility results in some degree of immobility. Contractility is impaired by many muscle diseases, random and engineered genetic mutations, and even healthy aging. Quantitative knowledge of the contractility of skeletal muscles is necessary to test hypotheses regarding the interactions between deficits in basic cellular and molecular processes and musculoskeletal frailty and immobility. Briefly, the specific aims of the Core are to provide for NSC investigators: (1) facilities, expertise, and technical support for measuring contractility of whole skeletal muscles and small strips of intact fibers from the diaphragm muscle (2) instruction and training in the knowledge and skills necessary to measure contractility; and (3) development of techniques and/or construction of specialized apparatus for the study of the contractility. Contractility is measured initially at the level of the whole skeletal muscle to provide the broadest spectrum of functional changes arising from cellular and molecular changes in skeletal muscle fibers due to aging or experimental interventions. At later stages in a project, more sophisticated evaluations may be required at the cellular or molecular levels. The Contractility Core was developed to ensure that all NSC investigators are able to include valid and reliable measurements of skeletal muscle contractility in their experiments. Consequently, the Contractility Core provides free to any NSC investigator: (1) one study of six pairs of control and experimental rodents; (2) assistance in education and training NSC investigators, their staff, or trainees to set-up the apparatus and measure contractility independently; and (3) assistance in the development of one innovative technique, or the construction of one specialized instrument for the measurement of contractility per year. Subsequent use of the Core is generally on a fee-for-service basis, but if results are promising and particularly if the NSC investigator proposes to seek outside funding, additional experiments may be supported by the Core budget from unallocated charge-back-fees. Through the provision of state-of-the-art facilities, techniques and expertise, the Core enhances and encourages multidisciplinary research in the basic biology of aging at UM and worldwide.

骨骼肌收缩性定义为肌肉中收缩蛋白相互作用并产生力的能力。所有的身体运动都依赖于骨骼肌的收缩。因此，骨骼肌收缩力的任何下降都会导致某种程度的不动。许多肌肉疾病、随机和工程基因突变，甚至健康衰老都会损害收缩力。骨骼肌收缩性的定量知识对于检验有关基本细胞和分子过程缺陷与肌肉骨骼虚弱和不动之间相互作用的假设是必要的。简而言之，核心的具体目标是为 NSC 研究人员提供：（1）用于测量整个骨骼肌和膈肌完整纤维小条的收缩性的设施、专业知识和技术支持（2）测量收缩力所需的知识和技能； (3) 开发用于研究收缩性的技术和/或建造专用设备。收缩性最初是在整个骨骼肌的水平上测量的，以提供由于衰老或实验干预而导致骨骼肌纤维的细胞和分子变化所引起的最广泛的功能变化。在项目的后期阶段，可能需要在细胞或分子水平上进行更复杂的评估。收缩性核心的开发是为了确保所有 NSC 研究人员能够在他们的实验中纳入有效且可靠的骨骼肌收缩性测量。因此，收缩性核心向任何 NSC 研究者免费提供：（1）一项针对六对对照和实验啮齿动物的研究； (2) 协助教育和培训 NSC 研究者、其工作人员或受训人员独立安装仪器并测量收缩力； (3) 每年协助开发一项创新技术，或建造一台用于测量收缩力的专用仪器。核心的后续使用通常是按服务收费的，但如果结果有希望，特别是如果国家安全委员会研究人员建议寻求外部资金，则核心预算可能会从未分配的退款费用中支持额外的实验。通过提供最先进的设施、技术和专业知识，该核心加强和鼓励密歇根大学和全世界在衰老基础生物学方面的多学科研究。