CORE--CONTRACTILITY

核心——收缩性

• 批准号: 6592107
• 负责人: JOHN Arthur FAULKNER
• 金额: $ 25.46万
• 依托单位: UNIVERSITY OF MICHIGAN AT ANN ARBOR
• 依托单位国家: 美国
• 财政年份: 2002
• 资助国家: 美国
• 起止时间: 2002-05-15 至 2003-04-30
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6592107
• 关键词: aging; biomedical facility; biotechnology; data collection methodology /evaluation; fiber cell; genetically modified animals; laboratory mouse; measurement; mechanical pressure; muscle contraction; muscular dystrophy; myofibrils; statistics /biometry; striated muscles

The purpose of the Contractility Core is to provide collaborating investigators in the Program Project with reliable and valid measures of the contractile properties of whole striated muscles and of strips of diaphragm muscle fibers, statistical analysis of the data, and interpretation of the structure-function relationships. The Core will also provide opportunities for instruction and training for faculty and trainees who wish to learn the techniques. Muscle fibers when activated attempt to contract or shorten. Whether an activated myofiber shortens, stays at the same length or is stretched depends on the interaction between the force developed and the load. For striated muscle, contractility is defined as the capability of muscle fibers to develop force during fixed length or isometric contractions, during shortening or miometric contractions, and during lengthening or pliometric contractions. Our working hypothesis is that contractility of striated muscles is a complex phenomenon and the provision of reliable and valid measurements requires sophisticated equipment and highly trained muscle mechanicists to make the measurements and analyze and interpret the results. Consequently, to test hypothesis relating the underlying mechanisms of the reduced contractility of striated muscles in diseased and old animals rigorously, a Contractility Core is a necessity. Impairments during each of the three types of contraction may occur at any age due to injury or disease or as an intractable concomitant of aging. The impairments in contractility, whether due to injury, disease, or old age, limit the activities of daily living and reduce the quality of life, particularly for the sick and the elderly.

收缩核心的目的是提供协作 项目研究人员采取可靠有效的措施 整个横纹肌和横纹肌条的收缩特性 膈肌纤维，数据统计分析，以及 结构-功能关系的解释。核心还将 为教师和人员提供指导和培训的机会 想要学习技术的学员。激活时的肌纤维 尝试收缩或缩短。激活的肌纤维是否缩短， 保持相同的长度或被拉伸取决于相互作用 产生的力和负载之间。对于横纹肌来说， 收缩性定义为肌纤维发育的能力 固定长度或等长收缩期间、缩短或 收缩，以及延长或增强收缩期间。 我们的工作假设是横纹肌的收缩性是 复杂的现象并提供可靠有效的测量 需要先进的设备和训练有素的肌肉机械师 进行测量并分析和解释结果。最后， 检验与减少的潜在机制有关的假设 患病动物和老年动物横纹肌的收缩性严格， 收缩核心是必要的。三个期间的减值 由于受伤或疾病或其他原因，任何年龄的宫缩都可能发生 衰老的顽固伴随物。收缩力受损， 无论是由于受伤、疾病还是年老，限制日常活动 生活并降低生活质量，特别是对于病人和病人 老年。