Development and validation of a Parallel Artificial Membrane Assay Permeation model for personal care formulations

个人护理配方平行人工膜测定渗透模型的开发和验证

• 批准号: 2870236
• 金额: --
• 依托单位: University College London
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2870236
• 关键词: Development; validation; Parallel; Artificial; Membrane

Excised human skin is the most preferred approach for the assessment of topical formulations using in vitro skin permeation studies. The major challenges in using excised human skin are (1) the difficulty in sourcing the skin samples and (2) intra- and inter-specimen variability (Dick and Scott, 1992; Schmook et al., 2001). Several alternative models have been developed for prediction of skin permeation for chemicals. One of these models, the skin Parallel Artificial Membrane Permeation Assay (PAMPA) model has been investigated for prediction of the skin permeation of several molecules. The skin PAMPA membrane is essentially a filter substrate coated with synthetic lipids arranged in 96-well plate and allows for rapid and high-throughput screening of compounds. Recently, the UCL group has performed comparative studies for permeation in the skin PAMPA membrane and in human tissue under finite dose conditions for caffeine, ibuprofen and niacinamide. Very good correlations were observed for cumulative amounts of active permeated over time in the PAMPA model compared with conventional in vitro permeation studies with animal tissue. However, it is important to note that to date, very simple formulations e.g. single or binary solvent systems have been investigated in the PAMPA model.

切除的人体皮肤是使用体外皮肤渗透研究评估局部制剂的最优选方法。使用切除的人体皮肤的主要挑战是（1）获取皮肤样本的困难和（2）样本内和样本间的变异性（Dick 和 Scott，1992 年；Schmook 等人，2001 年）。已经开发了几种替代模型来预测化学品的皮肤渗透性。其中一种模型，皮肤平行人工膜渗透测定 (PAMPA) 模型已被研究用于预测几种分子的皮肤渗透。皮肤 PAMPA 膜本质上是一种涂有合成脂质的过滤基质，排列在 96 孔板中，可以快速、高通量地筛选化合物。最近，UCL小组对咖啡因、布洛芬和烟酰胺在有限剂量条件下在皮肤PAMPA膜和人体组织中的渗透进行了比较研究。与传统的动物组织体外渗透研究相比，PAMPA 模型中随着时间的推移活性渗透的累积量观察到非常好的相关性。然而，值得注意的是，迄今为止，配方非常简单，例如单一或二元溶剂系统已在 PAMPA 模型中进行了研究。