Agilent 6890 GC/5973 mass detector for Profiling

用于分析的 Agilent 6890 GC/5973 质量检测器

• 批准号: 6877331
• 负责人: ROGER A DAVIS
• 金额: $ 11.68万
• 依托单位: SAN DIEGO STATE UNIVERSITY
• 依托单位国家: 美国
• 财政年份: 2005
• 资助国家: 美国
• 起止时间: 2005-04-05 至 2006-04-04
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/6877331
• 关键词: biomedical equipment purchase; gas chromatography mass spectrometry; lipid metabolism; liquid chromatography mass spectrometry; oxidized lipid

DESCRIPTION (provided by applicant): We are a group of investigators with long-term collaborations centering on our common interest in metabolic disease and atherosclerosis. The Davis lab's contribution to the technical expertise of the group has been to provide lipid quantitation and oxysterol analysis using gas/liquid chromatography and GC/mass spectroscopy. The purpose of this application is to seek funds to procure a GC/mass spectrometer allowing us to analyze cells, tissues and isolates for lipids, sterols and metabolites. In addition, this machine will enable us to perform stable isotope dilution analysis in order to assess how phenotype (i.e. the in vivo metabolism of lipids, sterols and metabolites) may be linked to the genetic background of specific mice. We request an Agilent 6890 GC having a split/splitless all glass injector connected to a flame ionization detector as well as a 5973 mass selective detector enabling us to quantitate cholesterol/sterols/fatty acids and to analyze the metabolic profiles of congenic and genetically altered mice as a means to link genomics with metabolic phenotypes with emphasis on susceptibility to atherosclerosis. This equipment is needed to replace the Davis lab's 19 year old Hewlett-Packard 5890A GC having a split/splitless all glass injector connected to a flame ionization detector as well as a 5970 mass selective detector. During the past 3 years, the Davis lab has spent over $8000 to have this instrument repeatedly fixed using salvaged parts. Unfortunately, this was a waste of time and money, as many parts to the machine were outdated and replacement not possible. We have now have been forced to agree with Agilent that our old machine can not be fixed in a reliable manner. It is for this reason that we seek funds to purchase a replacement GC/mass spectrometer. Each lab has used GC sterol analysis as shown in table 1. Because of our dependence on this technique, prior to breaking down we used the GC -60% of the time. The projected use of each investigator is shown in table 1 and will account for 100% time use. Therefore, we have no alternative equipment for this essential technique.

描述（由申请人提供）：我们是一群研究人员，他们长期合作以我们对代谢疾病和动脉粥样硬化的共同兴趣为中心。戴维斯实验室对该组技术专长的贡献是使用气相/液相色谱和GC/质谱法提供脂质定量和氧化酚分析。该应用的目的是寻求资金购买GC/质谱仪，使我们能够分析脂质，固醇和代谢物的细胞，组织和分离株。此外，该机器将使我们能够进行稳定的同位素稀释分析，以评估表型（即脂质，固醇和代谢物的体内代谢）如何与特定小鼠的遗传背景有关。我们要求具有与火焰电离探测器相关的所有玻璃注射器的分裂/无玻璃注射器以及5973个质量选择探测器，使我们能够定量胆固醇/固醇/脂肪酸，并分析与含量和遗传变化的幼体相关的胆固醇/固醇/脂肪酸，并将其与遗传型的现象分析，使我们能够定量胆固醇/固醇/脂肪酸，使我们能够定量胆固醇/固醇/脂肪酸，使我们能够定量胆固醇/固醇/脂肪酸，使我们能够定量胆固醇/固醇/脂肪酸，并将其与含量变化的鼠标联系在一起动脉粥样硬化。需要此设备来替换戴维斯实验室（Davis Lab）19岁的惠普（Hewlett-Packard）5890A GC，其拆分/无分玻璃将所有玻璃注射器连接到火焰电离探测器以及5970质量选择性检测器。在过去的三年中，戴维斯实验室花费了8000多美元，以用挽救的零件重复修复该乐器。不幸的是，这是浪费时间和金钱，因为机器的许多部分已经过时了，无法更换。现在，我们被迫同意安吉特人的同意，即我们的旧机器不能以可靠的方式固定。正是由于这个原因，我们寻求资金购买替换GC/质谱仪。每个实验室都使用GC固醇分析，如表1所示。由于我们对该技术的依赖，在分解之前，我们使用了GC -60％的时间。表1中显示了每个研究者的预计使用，并将占100％的时间使用。因此，我们没有此基本技术的其他设备。