Oxygen Gas Perfusion System for Pancreas Preservation

用于保存胰腺的氧气灌注系统

• 批准号: 7056605
• 负责人: LINDA A TEMPELMAN
• 金额: $ 24.99万
• 依托单位: GINER, INC.
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-04-01 至 2008-03-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7056605
• 关键词: diabetes mellitus; electrochemistry; laboratory mouse; pancreas; pancreatic islet transplantation; perfusion; portable biomedical equipment; swine; technology /technique development; tissue /organ preservation

DESCRIPTION (provided by applicant): The goal of the proposed work is to develop a substantially improved technique of preserving the human pancreas in order to improve the quality of the isolated human islets that are harvested from it for use in human islet transplantation for Type 1 diabetes. Currently, human pancreata are transported with the two layer method (TLM) of storage (8¿C), which uses an oxygenated perfluorocarbon solution as an external oxygen reservoir. While the TLM is widely reported to improve islet quality, there are still insufficiencies in the number of viable islets acquired. This is partially attributable to viable islet loss during pancreas storage. Preliminary calculations and experiments indicate that external oxygenation of the pancreas only benefits tissue in the outer rim of the organ leaving the majority of the volume of the pancreas without oxygenation (85-95%). The proposed work will use a novel method of oxygen delivery to the interior of the organ. Another innovative component of the research and development is to develop a system to generate and regulate oxygen delivery to the organ using a portable electrochemical oxygen concentrator/generator. The proposed electrochemical oxygen concentrator uses electrical power to concentrate oxygen from air and release it as pure, humidified oxygen that can be blended with ambient air to give the most efficacious pO2 for the proposed preservation technique. Delivery of gaseous oxygen to the interior of the organ is less complex than full liquid perfusion of the organ. Also the proposed system will be designed to be portable since the main "storage" time of the pancreas before islet isolation is during its transportation to a facility that specializes in harvesting islets from pancreata. Oxygen will be continuously generated at the desired flow rate and pressure without the need for transporting pressurized gas cylinders. This proposed method has the potential to effect a breakthrough, rather than an incremental improvement, in the quality of islets harvested from the pancreas. The effective treatment of serious cases of Type I Diabetes through pancreatic islet transplant is a high priority in cutting-edge healthcare research, and the proposed work will contribute to the advancement of the field. COMMERCIAL POTENTIAL The preservation system equipment could be a specialty commercial product, supplied to the major organ harvest and transplantation centers. Alternatively, the transportation and preservation of pancreata, using the proposed technique, could be a commercial service. The techniques and equipment could be applicable to the preservation of other organs. KEYWORDS pancreas, pancreata, Type I Diabetes, organ transport, islet transplantation, electrochemical oxygen concentration.

描述（通过应用程序证明）：支撑工作的目的是开发了保留人类palcress的技术，以提高从中收获的孤立人类胰岛的质量，以用于1型糖尿病的人类胰岛移植用两层方法（TLM）oftorage（8？）作为外部氧气储存，而TLM被广泛报道以改善胰岛质量，但在获取的Viablets数量中仍然存在。外部氧气的氧气在器官的外边缘中有益于大多数p骨的氧气，而没有氧气的氧气（85-95％）研发的发展是开发的，是使用便携式电化学氧气浓缩器生成和调节器官的氧气氧气是液体的主要“储存时间”。从胰腺上收获，通过胰腺移植是I型糖尿病的有效治疗，这是高优先级的n尖端医疗保健研究，而支撑的工作则可以使用pancreata aspecialty of pancement。支撑技术可能是商业服务。