Isolation and characterization of matrix bound vesicles for bone repair

用于骨修复的基质结合囊泡的分离和表征

• 批准号: 2794913
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2794913
• 关键词: Isolation; characterization; matrix; bound; vesicles

Extracellular matrix (ECM) derived biomaterials have been used in more than 8 million patients worldwide. Yet, the mechanisms by which ECM biomaterials modulate cell behaviour are not fully understood. Recent discovery of matrix bound vesicles in soft tissue ECM has provided a potential rationale for regulation of cell function, analogous to that observed with extracellular vesicles.This project will use multidisciplinary and cross-cutting approaches to enable the isolation and characterisation of matrix bound vesicles from bone tissue derived ECM biomaterials. The project will commence by optimising isolation methods to obtain matrix bound vesicles from bone. We will then define the aetiology of the isolated MBV population by comparison of the cytokine, protein and metabolic profiles using OrbiSIMS, lipidomics and imaging techniques. Defining the role of decellularized bone MBVs in the extracellular niche will inform our understanding of the biological impacts of bone derived MBVs. This in turn may enable the potential development of new therapeutics for bone repair.

全球超过800万患者使用了细胞外基质（ECM）衍生的生物材料。然而，ECM生物材料调节细胞行为的机制尚不完全了解。软组织ECM中基质结合囊泡的最新发现为调节细胞功能提供了潜在的理由，该基本与细胞外囊泡相似。该项目将采用多学科和跨基因方法来启用从骨组织衍生的ECM生物生物生物生物生物组织中的基质囊泡的分离和表征。该项目将通过优化隔离方法开始从骨头获得基质结合囊泡。然后，我们将使用Orbisims，脂肪组学和成像技术比较细胞因子，蛋白质和代谢谱，定义分离的MBV种群的病因。定义脱细胞骨MBV在细胞外生态位中的作用将为我们了解我们对骨衍生的MBV的生物学影响的理解。反过来，这可能使新的治疗剂的潜在发展用于骨修复。