Isolation and characterization of matrix bound vesicles for bone repair

用于骨修复的基质结合囊泡的分离和表征

• 批准号: 2794913
• 金额: --
• 依托单位: University of Nottingham
• 依托单位国家: 英国
• 项目类别: Studentship
• 财政年份: 2023
• 资助国家: 英国
• 起止时间: 2023 至 无数据
• 项目状态: 未结题
• 来源: https://gtr.ukri.org/projects?ref=studentship-2794913
• 关键词: Isolation; characterization; matrix; bound; vesicles

Extracellular matrix (ECM) derived biomaterials have been used in more than 8 million patients worldwide. Yet, the mechanisms by which ECM biomaterials modulate cell behaviour are not fully understood. Recent discovery of matrix bound vesicles in soft tissue ECM has provided a potential rationale for regulation of cell function, analogous to that observed with extracellular vesicles.This project will use multidisciplinary and cross-cutting approaches to enable the isolation and characterisation of matrix bound vesicles from bone tissue derived ECM biomaterials. The project will commence by optimising isolation methods to obtain matrix bound vesicles from bone. We will then define the aetiology of the isolated MBV population by comparison of the cytokine, protein and metabolic profiles using OrbiSIMS, lipidomics and imaging techniques. Defining the role of decellularized bone MBVs in the extracellular niche will inform our understanding of the biological impacts of bone derived MBVs. This in turn may enable the potential development of new therapeutics for bone repair.

细胞外基质 (ECM) 衍生的生物材料已被用于全球超过 800 万患者。然而，ECM 生物材料调节细胞行为的机制尚不完全清楚。最近在软组织 ECM 中发现的基质结合囊泡为细胞功能的调节提供了潜在的理论依据，类似于细胞外囊泡观察到的情况。该项目将使用多学科和交叉方法来分离和表征来自软组织 ECM 的基质结合囊泡。骨组织衍生的 ECM 生物材料。该项目将首先优化分离方法，从骨中获得基质结合的囊泡。然后，我们将使用 OrbiSIMS、脂质组学和成像技术通过比较细胞因子、蛋白质和代谢特征来定义分离的 MBV 群体的病因学。定义脱细胞骨 MBV 在细胞外生态位中的作用将有助于我们了解骨源性 MBV 的生物学影响。这反过来又可能促进骨修复新疗法的开发。