Fast Optical Imaging with Flavoprotein Autofluorescence in Visual Cortex

视觉皮层中黄素蛋白自发荧光的快速光学成像

• 批准号: 7139473
• 负责人: MITSUHIRO FUKUDA
• 金额: $ 21.22万
• 依托单位: UNIVERSITY OF PITTSBURGH AT PITTSBURGH
• 依托单位国家: 美国
• 财政年份: 2006
• 资助国家: 美国
• 起止时间: 2006-09-15 至 2008-08-31
• 项目状态: 已结题
• 来源: https://reporter.nih.gov/project-details/7139473
• 关键词: brain; brain mapping; cats; fasting; flavoproteins; human; orientation; visual cortex

DESCRIPTION (provided by applicant): In this research, we will focus on spatial and temporal resolution of the autofluorescence signal induced by neural activity. Optical imaging of autofluorescence signal of mitochondrial endogenous flavoprotein can be used for monitoring spatial and temporal patterns of neural activity from a relatively large cortical area of the brain with high spatial and temporal resolution. Despite a long history of optical measurements of cellular autofluorescence and recent successful attempts of flavoprotein imaging in rodent cortex in vivo, application of this technique to phylogenetically higher animals and humans for functional brain mapping has been underestimated. The specific aim of this proposed research is to investigate the feasibility of flavoprotein autofluorescence imaging for functional brain mapping in phylogenetically higher animals' visual cortex whose functions are relevant to the human brain. Our long-term goal is to elucidate the spatiotemporal correlation between neuronal activity and their metabolic responses induced by stimulation, and inherent spatial and temporal resolution of the metabolic-based functional brain imaging. The specific hypotheses to be tested are: 1) neural activity-dependant metabolic change detected by flavoprotein autofluorescence signal is highly confined to neural active sites, and 2) the flavin autofluorescence signal immediately emerges after the onset of stimulation. We base these hypotheses on general observations that i) a stimulus-induced increase in gluclose utilization is well localized to neural active sites and that ii) a stimulus- induced tissue oxygen tension change is tightly correlated with neural spiking activity. We will test these hypotheses on the model of orientation columns in the primary visual cortex. The orientation column is a functional structure related to stimulus orientation, in which neurons in response to the same orientation cluster together and form columns through pia to white mater, and it has been extensively investigated electrophysiologically, anatomically, and with hemodynamic-based optical imaging of intrinsic signals. The orientation maps obtained from flavoprotein signal will be quantitatively compared to the maps of the well- established hemodynamic-based intrinsic signals for its evaluation. We believe that the establishment of this technique will greatly improve the acuracy of mapping neural activity which will provide cruicial information for diagnosis and treatment of disease and human brain functions.

描述（由申请人提供）： 在这项研究中，我们将重点关注神经活动引起的自荧光信号的空间和时间分辨率。线粒体内源性黄蛋蛋白的自荧光信号的光学成像可用于监测来自大脑相对较大的具有高空间和时间分辨率的神经活动的空间和时间模式。尽管在体内的啮齿动物皮质中，在啮齿动物皮质中进行了较长的细胞自动荧光光学测量和最近成功尝试的尝试，但已低估了该技术在系统发育上的较高动物和人类用于功能性脑图上的尝试。这项拟议的研究的具体目的是研究黄素蛋白自动荧光成像的可行性，用于在较高的动物视觉皮层中具有与人脑相关的系统发育较高动物的视觉皮层。我们的长期目标是阐明神经元活性与刺激引起的代谢反应之间的时空相关性，以及基于代谢的功能性脑成像的固有空间和时间分辨率。要测试的特定假设是：1）通过黄素蛋白自动荧光信号检测到的神经活动依赖性代谢变化，高度局限于神经活性位点，2）刺激开始后立即出现了黄素自动荧光信号。我们将这些假设基于一般观察结果，即i）刺激诱导的葡萄糖利用率的增加与神经活性部位良好，并且ii）ii）刺激诱导的组织氧张力的变化与神经尖峰活性密切相关。我们将在主要视觉皮层的方向列模型上测试这些假设。取向柱是与刺激方向相关的功能结构，其中神经元响应相同的取向簇一起，并通过PIA形成对白色母乳的柱形成柱，并且在解剖学上进行了广泛研究，并在解剖学上进行了广泛的研究，并且基于血液动力学的基于固有信号的光学动力学成像。与良好的基于​​血液动力学的内在信号的图相比，从黄蛋白信号获得的取向图将进行定量。我们认为，这项技术的建立将大大改善映射神经活动的可观性，这将为诊断和治疗疾病和人脑功能提供巡航信息。